| 氟对原代培养大鼠肝细胞的氧化损伤作用研究 |
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| 引用本文： | 郭晓英,孙贵范.氟对原代培养大鼠肝细胞的氧化损伤作用研究[J].中国地方病防治杂志,2005,20(3):129-131. |
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| 作者姓名： | 郭晓英 孙贵范 |
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| 作者单位： | 中国医科大学公共卫生学院流行病学教研室,沈阳,110001 |
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| 摘 要： | 目的探讨氟对原代培养大鼠肝细胞的氧化损伤作用。方法采用半原位酶消化法分离大鼠肝细胞，氟化物染毒24h后用噻唑蓝(MTT)比色法检测细胞存活率，检测培养液中谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性及脂质过氧化产物丙二醛(MDA)的含量。结果氟对大鼠肝细胞具有明显的毒性作用，表现为细胞存活率下降，ALT、AST的活性升高，MDA含量增加。结论脂质过氧化引起的氧化损伤作用是氟致原代培养大鼠肝细胞毒性的主要原因。
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| 关 键 词： | 大鼠肝细胞 原代培养 作用研究 谷丙转氨酶（ALT） 丙二醛（MDA） 氧化损伤作用 脂质过氧化产物 细胞存活率 谷草转氨酶 MDA含量 肝细胞毒性 酶消化法 毒性作用 比色法 噻唑蓝 24h 氟化物 培养液 AST 检测 活性 |
| 文章编号： | 1001-1889(2005)03-0129-03 |
| 修稿时间： | 2005年2月2日 |
Study of fluoride on oxidative damage in primary cultured rat hepatocytes |
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| GUO Xiao-ying,SUN Gui-fan.Study of fluoride on oxidative damage in primary cultured rat hepatocytes[J].Chinese Journal of Control of Endemic Disenaces,2005,20(3):129-131. |
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| Authors: | GUO Xiao-ying SUN Gui-fan |
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| Abstract: | Objective To study the oxidative damage after fluoride exposure in primary cultured rat hepatocytes. Methods Hepatocytes were isolated by half-in situ perfusion method and cultured with various concentrations of sodium fluoride for 24 hours. MTT assays were performed to test cell viability. Meanwhile the activities of ALT, AST and content of MDA were determined. Results Fluoride was toxic to rat hepatocytes. The cell viability decreased. The activities of ALT, AST and the content of MDA increased. Conclusions Oxidative damage induced by lipid peroxidation was the main reason of the cytotoxicity of rat hepatocytes. |
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| Keywords: | Sodium Fluoride Oxidative Damage Primary Cultured Hepatocyte |
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