柱切换HPLC法测定豚鼠体内诺氟沙星银解离物诺氟沙星的浓度

用柱切换HPLC法建立了豚鼠血浆和皮下组织液中诺氟沙星银体内主要解离物诺氟沙星的测定方法。预处理柱为μ-BondapakC₁₈,37～50μm,50mm×5mmID;分析柱为YWG-C₁₈,10μm,150mm×5mmID。预处理流动相为0.008mo1/L磷酸缓冲液,流速为3ml/min;分析流动相为甲醇—0.008mol/L磷酸缓冲液—0.05mol/L四丁基溴化铵(25:75:4)。紫外检测波长为280nm。皮下组织液及血浆中的线性范围分别为100～3200ng/ml(r=0.9999)和2～128μg/ml(r=0.9999);最低检测浓度分别为10ng/ml和0.25μg/ml,方法的平均回收率为102%,日内及日间偏差均小于7%。

DETERMINATION OF NORFLOXACIN, AN ACTIVE DISSOCIATIONPRODUCT OF SOLVER NORFLOXACIN,IN GUINEA PIG PLASMA AND TISSUE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH COLUMN-SWITCHING

A column switching HPLC method was established for the determination ofnorfloxacin, and active dissociation product of silver norfloxacin ,in guinea pig plasma and tissues.The pre-treating column(50 mm×5mm ID)and analytical column( 150 mm×5mm ID )werepacked with μ-Bondapak C₁₈ ,37～50μm and YWG- C₁₈, 10μm,respectively. A mixture ofCH₃OH—0. 008 mol/L phosphate buffer—.05 mol/L tetrabutylammoniun bromide(25 :75 :4)was used as the analytical mobile phase with a flow rate of 1 ml/min. The pre-treating mobile phasewas 0.008 mol/L phosphate buffer with a flow rate of 3 ml/min. UV detection was made at 280 nm.The results were linear within a range of 2～128μg/ml norfloxacin for plasma,The lowest detectionlimit was 0.25μg/ml for plasma. The mean recovery of the method was 102%with a relativestandard deviation of less than 7%.