| 反相高效液相色谱法测定牛黄类中成药中胆汁酸的含量 |
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| 引用本文： | 倪坤仪,王建,陈健,郁建,屠树滋. 反相高效液相色谱法测定牛黄类中成药中胆汁酸的含量[J]. 药学学报, 1994, 29(8): 624-633 |
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| 作者姓名： | 倪坤仪 王建 陈健 郁建 屠树滋 |
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| 作者单位： | 中国药科大学，浙江省药品检验所，成都军区军医学校 |
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| 摘 要： | 反相高效液相色谱法测定牛黄类中成药中胆汁酸的含量倪坤仪，王建，陈健，郁建，屠树滋（中国药科大学２１０００９）含牛黄的中成药种类很多，在医疗中具有广泛的用途。中药牛黄中主要成分为胆汁酸和胆红素。本文主要研究用ＨＰＬＣ法测定牛黄以及含牛黄中成药中胆汁酸的...
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| 关 键 词： | 反相高效液相色谱法 六神丸 六应丸 牛黄消炎丸 胆汁酸 |
| 收稿时间： | 1993-12-20 |
DETERMINATION OF BILE ACIDS IN BEZOAR AND CHINESE PATENT MEDICINES CONTAINING BEZOAR BY REVERSED PHASE HPLC |
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| KY Ni,J Wang,J Chen,J Yu and SZ Tu. DETERMINATION OF BILE ACIDS IN BEZOAR AND CHINESE PATENT MEDICINES CONTAINING BEZOAR BY REVERSED PHASE HPLC[J]. Acta pharmaceutica Sinica, 1994, 29(8): 624-633 |
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| Authors: | KY Ni J Wang J Chen J Yu SZ Tu |
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| Abstract: | A high performance liquid chromatograbphic method was established for the deter-mination of cholic acid and hyodeoxycholic acid in bezoar, Liushen pills ,Liuying pills and Niuhuang Xiaoyan pills. Bile acids were labelled with 1-bromoacetylnitrobenzene in acetonitrile using 18-crown- 6 as catalyst. Derivatized bile acids were separated and determined on uBondapak C18column (3.9mm×300mm,8～10um),using methanol-acetonitrile -water(24:35:41) as mobile phase and mandrolone phenylpropionate as internal standard. Standard .Selection of condition of derivatization reaction, mobile phase optimization, determination of linearity range and check of intervenient factor have been made.The accuracy of the method was checked by recoveries. |
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| Keywords: | Reversed phase HPLC Liushen pills:Liuying pills: Niuhuang Xiaoyan pills: Bile acids |
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