瘫立定对脊髓损伤大鼠运动功能和iNOS的影响

目的：观察瘫立定对脊髓损伤（SCI）大鼠模型双后肢运动功能及脊髓组织中诱导型一氧化氮合酶（iNOS）阳性细胞率变化的影响。方法：60只SD大鼠随机分为6组，用改良的Allen’s法造模，分别应用地塞米松、瘫立定灌胃，于伤后4h、8h、1d、3d、7d，采用斜板试验测定大鼠双后肢运动功能；用免疫组化方法检测脊髓组织 iNOS 阳性细胞率的变化。结果：伤后7d，瘫立定高剂量组大鼠斜板实验角度为47.6°±3.43°，与地塞米松组48.6±3.64°比较，无统计学意义（P>0.05），两者作用相当。伤后3～7d，瘫立定高剂量组iNOS阳性细胞率42.06%±2.67%，与地塞米松组43.10%±2.39%比较，无统计学意义（P>0.05），两者作用相当。结论：瘫立定对急性脊髓损伤运动功能恢复有促进作用；能有效抑制大鼠脊髓损伤后iNOS的表达。

Effect of Tanliding on movement function and iNOS in rats modeled with SCI

Objective： To observe the effect of Tanliding （TLD） on rats＇movement function and on positive cells ratio of induced nitric oxide synthase （iNOS） of rats modeled with spinal cord injury （SCI）. Methods： 60 rats were randomly divided into six groups. All rats were injured with Allen＇s weight-dropping method. Then Dexamethasone and TLD were given individually to corresponding rats by gavage. Rats＇ movement fimction was determined through the Leaned-board experiment （tilt table test） at 4h, 8h, 24h, 3d and 7d after injury. Positive cells ratio ofiNOS was determined at the same time-points. Results： At the 7th day, the degree of angle in tilt table test is 47.6°± 3.43° in the group of high dose of TLD, compared with 48.6° ±3.64° in Dexamethasone group, there is no significant difference （P〉0.05）. The rates of positive cells ofiNOS in TLD and Dexamethasone group were 42.06%±2.67% and 43.10%±2.39%, there is no significant difference （P〉0.05）. Conclusion： TLD can significantly improve movement function after SC1 and inhibit expression of iNOS.