氨基胍对糖尿病大鼠一氧化氮一氧化氮合酶及尿蛋白的影响

目的探讨氨基胍(AG)对早期糖尿病(DM)大鼠NO、一氧化氮合酶(NOS)活性及24 h尿蛋白排泄量(24 hUPE)的影响。方法选择健康清洁级Wistar大鼠40只,检测24 hUPE、血清NO、总一氧化氮合酶(tNOS)和诱导型一氧化氮合酶(iNOS)及结构型一氧化氮合酶(cNOS)活性等5项指标后,用链脲佐菌素(STZ)60 mg/kg制备成糖尿病大鼠模型,将其随机分为对照组和氨基胍组(AG组)。于8周末时再检测大鼠的上述5项指标并进行统计分析。结果对照组在8周末时24 hUPE、NO和iNOS均高于造模前(P<0.01或P<0.05),AG组:24 hUPE高于造模前(P<0.01),tNOSi、NOS、cNOS低于造模前(P<0.01或P<0.05)。8周末时AG组5项指标均低于对照组(P<0.05)。结论糖尿病患者早期应用AG可通过降低血NOS活性、NO生成量及其它机制,使24 hUPE减少,减轻肾脏的损害。

EFFECTS OF AMINOGUANIDINE ON NITRIC OXIDE SYNTHASE AND URINARY PROTEIN EXCRETION OF DIABETIC RATS

Objective To investigate the effect of aminoguanidine(AG) on serum nitric oxide(NO),nitric oxide synthase(NOS) activity and renal function of diabetic rats.Methods Forty healthy Wistar rats were involved in the study.The 24-hour urinary protein excretion(24 hUPE),serum levels of NO,total nitric oxide synthase(tNOS) activity,inducible nitric oxide synthase(iNOS) activity and constructive nitric oxide synthase(cNOS) activity were detected.Streptozotocin(STZ,60mg/kg) was administrated in the rats.The STZ diabetic rats were randomly divided into diabetes control group and AG group.At the end of 8th week after STZ administration,the above five indexes of the rats were detected and statistical analysis was carried out.Results Compared with normal rats,24 hUPE,iNOS and NO of the rats in DM control group increased significantly(P<0.01,P<0.05),In the rats of AG group,24 hUPE increased while tNOS,iNOS and cNOS decreased(P<0.01,P<0.05).Compared with the diabetes control group,the five indexes significantly decreased in AG group(P<0.05).Conclusion In the progress of DM,the early administration of aminoguanidine can effectively reduce the 24-hour urinary protein excretion and renal injury by decreasing the NOS activity,NO level and other more important mechanisms.