| 人arresten因子对内皮细胞与LOVO细胞黏附的抑制 |
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| 引用本文： | 龙淼云,郑启昌,宋自芳,胡青钢,陈立波.人arresten因子对内皮细胞与LOVO细胞黏附的抑制[J].中华肿瘤防治杂志,2006,13(24):1846-1848. |
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| 作者姓名： | 龙淼云 郑启昌 宋自芳 胡青钢 陈立波 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院肝胆外科,湖北,武汉,430022 |
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| 基金项目： | 国家自然科学基金(30271242、30371396) |
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| 摘 要： | 目的:观察人arresten因子对人脐静脉内皮细胞HUVEC与LOVO细胞黏附的影响,探讨与之相关的黏附分子表达的变化。方法:半定量RT-PCR检测arresten对人脐静脉内皮细胞表达血管细胞黏附分子-1(VCAM-1)mrRNA的影响,Western-hlot检测VCAM-1蛋白表达的变化。孟加拉玫瑰红活细胞染色法观察arresten因子对内皮细胞与LOVO细胞黏附的影响。结果:RT-PCR及Western-blot结果表明VCAM-1在HUVEC细胞中高表达,但经过arresten因子处理的HUVEC细胞,其VCAM-1在mRNA水平及蛋白水平的表达均显著下降(P< 0．05)。孟加拉玫瑰红活细胞染色法显示arresten能抑制HUVEC细胞与LOVO细胞的黏附。结论:arresten因子通过降低内皮细胞VCAM-1的表达抑制内皮细胞与肿瘤细胞的黏附,这可能是其抑制肿瘤转移的一种途径。
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| 关 键 词： | 结肠肿瘤／病理学 脐静脉／细胞学 内皮 血管／细胞学 血管生成抑制剂／生物合成 细胞粘附分子／血液 |
| 文章编号： | 1673-5269（2006）24-1846-03 |
| 收稿时间： | 2006-05-24 |
| 修稿时间： | 2006-07-17 |
Anti-adhesion of HUVEC to LOVO cell induced by human arresten |
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| LONG Miao-yun,ZHENG Qi-chang,SONG Zi-fang,HU Qing-gang,CHEN Li-bo.Anti-adhesion of HUVEC to LOVO cell induced by human arresten[J].Chinese Journal of Cancer Prevention and Treatment,2006,13(24):1846-1848. |
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| Authors: | LONG Miao-yun ZHENG Qi-chang SONG Zi-fang HU Qing-gang CHEN Li-bo |
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| Institution: | Department of Hepatobiliary Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,P. R. China |
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| Abstract: | OBJECTIVE:To investigate anti-adhesion of HUVEC to LOVO cell induced by human arresten and measure the expression of VCAM-1 in HUVEC. METHODS: The expression of VCAM-1 in HUVEC lines was measured by semi- quantitative RT-PCR and Western blot, respectively. The adherence of HUVEC cell line to LOVO cell line was detected by using the rose bengal strain. RESULTS: The expression of VCAM-1 both at mRNA level and protein level decreased significantly in HUVEC cell line induced by human arresten (800 ng/mL)(P<0. 05). Human arresten (800ng/mL) inhibited the adherence of HUVEC cell line to LOVO cell line (P<0. 05). CONCLUSIONS: Human arresten inhibits the adherence of HUVEC cell line to LOVO cell line by inhibiting the expression of VCAM-1 in HUVEC cell line, and it may be used to prevent metastasis. |
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| Keywords: | colonic neoplasms/pathology umbilical veins/cytology endothelium vascular/cytology angiogenesis inhibitors/biosynthesis cell adhesion molecules/blood |
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