脓毒症大鼠血清炎症递质水平的变化

目的：探讨脓毒症大鼠血清炎症递质的变化。方法将30只雄性 SD 大鼠随机分为假手术组10只和脓毒症组20只，采用盲肠结扎穿孔法制作脓毒症模型。采集假手术组造模后颈总动脉血，采集脓毒症组造模后0、24、48、72 h 颈总动脉血，用酶联免疫吸附法检测血清肿瘤坏死因子α（TNF-α）、白细胞介素1（IL-1）、白细胞介素6（IL-6）的浓度。结果假手术组血清 TNF-α为（9.27±3.12）ng/ L，脓毒症组造模后0、24、48、72 h 分别为（9.26±8.01）、（32.01±4.52）、（55.22±7.61）、（83.31±8.57） ng/ L，脓毒症组造模后24、48、72 h 与造模后0 h 和假手术组比较差异均有统计学意义（P 均＜0.01）；假手术组血清 IL-1为（8.93±1.26）ng/ L，脓毒症组造模后0、24、48、72 h 分别为（20.01±3.51）、（25.51±2.79）、（59.67±3.26）、（87.86±11.51）ng/ L，脓毒症组造模后24、48、72 h 与造模后0 h 和假手术组比较差异均有统计学意义（P 均＜0.01）；假手术组血清 IL-6为（12.36±3.25）ng/ L，脓毒症组造模后0、24、48、72 h 分别为（11.52±2.32）、（31.59±12.12）、（57.27±13.53）、（71.59±12.67）ng/ L，脓毒症组造模后24、48、72 h 与造模后0 h 和假手术组比较差异均有统计学意义（P 均＜0.01）。结论脓毒症大鼠血清炎症递质水平明显升高，提示机体过度释放大量炎症递质，可能是导致脓毒症发生的因素之一。

The change of inflammatory mediators in the blood of septic rats

Objective To investigate the changes of serum inflammatory mediators in septic rats. Methods Thirty male SD rats were randomly divided into sham operation group（10 rats）and sepsis group （20 rats）. The model of sepsis was made by cecal ligation and puncture. Carotid artery blood samples were taken from the sham operation group,and from sepsis group at 0,24,48,72 h after model establishment. Enzyme linked immunosorbent assay（ELISA）was applied to detect the content of serum tumor necrosis factor alpha（TNF α）, interleukin 1（IL-1）,interleukin element 6（IL-6）. Results Serum TNF-α content in the sham operation group was（9. 27 ± 3. 12）ng/ L,and（9. 26 ± 8. 01）,（32. 01 ± 4. 52）,（55. 22 ± 7. 61）,（83. 31 ± 8. 57）ng/ L respectively at 0,24,48,72 h after model establishment in sepsis group. There were significant difference between sham operation group and sepsis group at different time points（P 〈 0. 01）. Serum IL-1in sham operation group was（8. 93 ± 1. 26）ng/ L,lower than that in sepsis group model at 0,24,48,72 h after model establishment （（20. 01 ± 3. 51）,（25. 51 ± 2. 79）,（59. 67 ± 3. 26）,（87. 86 ± 11. 51）ng/ L respectively）,and the differences were significant（P 〈 0. 01）. The serum IL-6 level in sepsis group at 0,24,48,72 h after model establishment were（11. 52 ± 2. 32）,（31. 59 ± 12. 12）,（57. 27 ± 13. 53）,（71. 59 ± 12. 67）ng/ L,respectively,different from that of sham operation group（（12. 36 ± 3. 25）ng/ L,（P 〈 0. 01））. Conclusion The serum transmitter levels in septic rats significantly increase,which suggest that a lot of inflammatory mediators are released,and it may be one of the factors for the occurrence of sepsis.