电针“足三里”对胃粘膜损伤大鼠Bcl-2、Fas蛋白表达的影响

目的 :观察电针“足三里”对胃粘膜损伤大鼠的细胞保护作用与细胞凋亡调控基因Bcl 2、Fas蛋白表达水平的关系及“足三里”是否具有腧穴相对特异性。方法 :40只大鼠 ,随机分为四组 ,即“足三里”组、非穴组、模型组和空白组 ,每组 1 0只。用无水乙醇按每只 1mL灌胃 ,造成胃粘膜损伤模型。各组检测胃粘膜细胞Bcl 2、Fas蛋白表达水平。结果 :Bcl 2基因蛋白阳性表达在“足三里”组非常明显 ,“足三里”组与模型组比较 ,有明显差异 (P <0 0 5)。Fas基因蛋白阳性表达在“足三里”组不明显 ,“足三里”组与非穴组、模型组比较 ,有极显著差异 (P <0 0 1 )。结论 :电针“足三里”对胃粘膜损伤后的细胞保护作用可能的机理 ,是由于促进了Bcl 2基因蛋白表达 ,抑制了Fas基因蛋白表达 ,并具有腧穴相对特异性。

Effect of Electroacupuncture of "Zusanli" on Expression of Bcl-2 and Fas Proteins in Alcohol-induced Gastromucosal Lesion Rats

Objective:To observe the effect of electroacupuncture (EA) of “Zusanli(ST 36) on expression of Bcl 2 and Fas proteins in alcohol induced gastromucosal lesion rats for analyzing the relationship between cytoprotection and expression of Bcl 2 and Fas proteins, and the point relative specificity.Methods:40 Wistar rats were randomly and evenly divided into “Zusanli"(ST 36),non acupoint, model and control groups. Gastric mucosal injury model was induced by intragastric infusion of dehydrated alcohol (1 mL per animal). The expression of Bcl 2 and Fas proteins in each group were detected with immunohistochemical techniques.: Expression of Bcl 2 protein of gastric mucosa tissues in “Zusanli" group was obviously stronger than that of model group( P <0 05 ).Expression of Fas protein in “Zusanli" group was decreased significantly in comparison with model and non acupoint groups ( P <0 01), suggesting that acupuncture of “Zusanli" generated up regulation of Bcl 2 expression and down regulation of Fas expression may contribute to its effect in protecting the gastromocosal tissue from injury and the effect of acupuncture of “Zusanli" is of relative specificity.:Electroacupuncture of “Zusanli"(ST 36) can up regulate expression of Bcl 2 and down regulate expression of Fas protein of gastromucosal tissues and the effect of “Zusanli"(ST 36) possesses a relative specificity.