无细胞壁细菌的分离培养

目的　观察普通血液培养中变异无细胞壁细菌 (L型细菌 )的生长现象 ,以提高血培养的阳性率。方法　对在普通血液培养过程中瓶底出现轻微暗紫红、上清液微混或瓶壁有少量颗粒状物生长时 ,分别用血浆分离针抽取可疑培养物同时转种于普通血液琼脂培养基、L型增菌培养基及L型琼脂培养基中培养 ,观察其生长情况。结果　从 2 0 0例普通血液培养瓶中分离出各种普通细菌 31例 (15 .5 % ) ,将可疑培养物再用血浆分离针转种于L型细菌液体培养基上又分离出 2 3例L型细菌 ,使原来一般血培养阳性率提高到2 7.0 %。结论　普通血培养过程中L型细菌生长时应及时、仔细观察和转种 ,才能提高L型细菌的检出率。

Separation cultivation of bacteria with no cell walls

Objective To observe the growth of bacteria with no cell walls(L type bacteria) in normal blood cultivation and increase its positive rate.Methods During normal blood cultivation,something a little dull red on the bottom of the bottle,a bit cloudy supernatant fluid or a few particles on the bottle walls occurred,and with culture plasma separating needles,suspected culture was extracted and implanted in normal plasma agar medium,L type liquid medium and L type agar medium,the the growth was observed.Results From 200 normal blood cultivation bottles,normal bacteria were separated in 31 ones(15.5 %),then the separated culture was implanted in L type liquid medium and L type bacteria were separated from 23 bottles,thus the positive rate of cultivation was increased to 27.0 %.Conclusion During normal blood cultivation,in time careful observation and implantation of L type bacteria can increase its detectable rate.