A Combined Molecular Approach to Screen for mec Gene Variants from Methicillin-resistant Staphylococcus aureus |
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| Affiliation: | 1. Department of Pathology (MA, JWS), University of Louisville Hospital, Louisville, Kentucky, USA;2. Department of Biology (MA, MHP), University of Louisville, Louisville, Kentucky, USA.;5. Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India;1. Department of Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;2. Department of Microbiology, Christian Medical College, Vellore, Tamil Nadu, India;3. Department of Microbiology, All India Institute of Medical Sciences, India;4. Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi, India;1. Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, India;2. P D Hinduja Hospital & Medical Research Centre, Mumbai, India;3. Jupiter Hospital, Pune, India;4. Clinical Microbiology & Immunology, Sir Ganga Ram Hospital, New Delhi, India;5. Infectious Diseases and Infection Control at Gleneagles Global Hospitals (Chennai, Bangalore, Hyderabad), India;6. Apollo Hospitals, Greams Road, Chennai, India;7. Metro Respiratory Center Pulmonology & Sleep Medicine, Metro Hospital, Noida, India;8. Emergency & Critical Care, S. G. P. G. I. M.S, Lucknow, India;9. Critical Care Medicine, Department of Academics & Health Research, AMRI Hospitals, Kolkata, India |
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| Abstract: | Previous reports have suggested a common origin for all methicillin resistance (mec) genes from methicillin-resistant Staphylococcus aureus (MRSA) isolates examined so far. The purpose of this study was to explore several molecular methods for screening MRSA isolates from different sources and, in some cases, with varying phenotypes. Eighty MRSA isolates from three teaching hospitals in the University of Louisville Medical Center were compared with MRSAs from a hospital in southern California and with methicillin-sensitive S. aureus isolates. The methods were used to detect the presence of mec gene and to screen for any polymorphisms in these genes for the respective strains. The mec gene for each isolate was amplified via the polymerase chain reaction, and each polymerase chain reaction product was compared to the others by restriction enzyme digestion, denaturing-gradient gel electrophoresis, and mutation detection enhancement. By these criteria, the mec genes from the 80 MRSA strains in this study seemed to be identical. Such a finding was not unexpected and supported the existing hypothesis of a common ancestor for all mec genes isolated in MRSA isolates. However, the combination of methods used in this study may facilitate screening of MRSA strains in population studies as mec gene variants begin to emerge. |
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