血塞通软胶囊对心肌梗死大鼠血流动力学及心肌细胞凋亡的影响

目的：观察血塞通软胶囊对大鼠心肌梗死后不同时间血流动力学及不同区域心肌细胞凋亡的影响。方法：采用结扎大鼠冠状动脉左前降支的方法建立心肌梗死模型，将大鼠分为假手术组、模型组、血塞通组。治疗48h和5周时，采用超声心动图检测左室射血分数和左室短轴缩短分数，多导生理记录仪检测主动脉和左心室血流动力学改变，病理切片观察心肌病理学改变，外源性荧光标记的脱氧核苷酸末端转移酶介导的dUTP缺口末端标记法检测不同区域心肌组织中的心肌细胞凋亡情况。结果：与模型组比较，心肌梗死后各个时期，血塞通组左室射血分数和左室短轴缩短分数均显著升高，心肌细胞凋亡指数下降（P〈0．01，P〈0．05）。心肌梗死后48h，血塞通组左室收缩压、左心室压力最大上升速率有显著升高，而心脏质量指数、左室舒张末压显著降低，与模型组比较差异有统计学意义（P〈0．01）；心肌梗死5周后，血塞通组左室舒张末压明显降低，左心室压力增高率明显升高，与模型组比较差异有统计学意义（P〈0．01）。结论：血塞通软胶囊可能通过改善心肌梗死后心功能，抑制心肌细胞凋亡从而有效治疗和预防心肌梗死后的心肌重塑和心室重构。

Effects of Xuesaitong Soft Capsule on hemodynamics and cardiocyte apoptosis of rats after myocardial infarction

Objective： To observe the effects of Xuesaitong Soft Capsule, a compound traditional Chinese medicine, on hemodynamics and cardiocyte apoptosis of rats after myocardial infarction （MI） in different time and areas, and to explore its mechanism in inhibiting cardiac ventricle reconstitution and muscle remodeling. Methods： Except rats in sham-operated group, MI was induced by ligaturing the left coronary artery main stem of rats＇ hearts. Then, rats were divided into untreated group and Xuesaitong group. Ejection fraction （EF） and fractional shortening （FS）, hemodynamics, ratio of heart weight to body weight （HW/BW ratio）, and pathological changes of cardiac tissue were analyzed to assess cardiac function and myocardial hypertrophy status 4,8 hours and 5 weeks after MI. Cardiocyte apoptosis in different areas was detected by fluorescently labeled TdT-mediated dUTP-biotin nick end labeling （TUNEL） and DNA ladder method.Results： Comparing with the untreated group, EF and FS in the Xuesaitong group were significantly increased. and the apoptosis index was significantly decreased （P〈0.01）; 48 hours after MI, left ventricular systolic pressure and maximum rate of left ventricular pressure rise were obviously increased, while left ventricular end-diastolic pressure （LVEDP） and heart weight to body weight ratio were decreased as compared with the untreated group （P〈0.01）; LVEDP in the Xuesaitong group was decreased, while maximum rate of left ventricular pressure rise and fall were increased as compared with the untreated group 5 weeks after MI （P,0.01）. Conlusion： Xuesaitong can efficiently treat reoonstitution and remodeling of cardiac ventricle of rats after myocardial infarction by improving the heart function and inhibiting the cardiocyte apoptosis.