"屋翳"穴与多穴针刺对乳腺增生病模型大鼠乳腺腺体的影响

目的:以乳腺增生病病理形态计量学为标准,筛选治疗乳腺增生病的关键穴位。方法:将Wistar未孕雌性大鼠48只随机分为正常组、模型组、单穴(屋翳)组、多穴组。除正常组外,以苯甲酸雌二醇0.5 mg/kg每日腹腔注射,25 d后改为每日腹腔注射黄体酮4 mg/kg,连续5 d,复制乳腺增生病模型。单穴组取“屋翳”穴,多穴组取“屋翳”“膻中”“合谷”“足三里”穴,每日针刺1次,留针5 min,9次为1疗程,共针刺3个疗程。治疗结束后取大鼠第2对乳房做病理切片,HE染色,镜下观察;美国IPP图像分析软件分析腺泡腔直径及面积。结果:模型组乳腺导管扩张,腺泡腔直径及面积均较正常组增大(均P<0.01);单穴组、多穴组腺泡腔直径及面积较模型组减小(P<0.01);单穴组与多穴组腺泡腔直径及面积无显著性差异(P>0.05)。结论:针刺“屋翳”穴可明显抑制雌激素注射引起的乳腺增生,说明“屋翳”穴是治疗乳腺增生的关键穴位。

Observation on the Effect of Acupuncture of "Wuyi" (ST 15) and Multiple Acupoints on the Size of Lacteal Gland in Rats with Cyclomastopathy

OBJECTIVE: To compare the therapeutic effects of acupuncture of "Wuyi" (ST 15) and multi-acupoints for hyperplasia of mammary glands so as to choose the key acupoints for cyclomastopathy. METHODS: A total of 48 FEMALE Wistar rats were randomized into normal control, model, "Wuyi" (ST 15) and multi-point groups with 12 cases in each group. Cyclomastopathy model was established by intraperitoneal injection of benzestrofol (0.5 mg/kg per day) for 25 days. followed by administration of flavolutan (4 mg/kg per day, i.p.) for 5 days. Bilateral ST15 were punctured and stimulated manually for ST15 group; while for multi-point group, bilateral ST15, "Hegu" (LI 4), "Zusanli" (ST 36) and "Shanzhong" (CV 17) were punctured, once daily, 27 sessions altogether. After decapitation, the rats' second pairs of mammary glands were removed to be fixed routinely, embedded in paraffin, sectioned (5 microm), and stained with H & E method for observing the structural changes under microscope and with norphometry. RESULTS: Compared with control group, the diameter and area of acinar lumina (AL) of mammary glands in model group increased significantly (P < 0.01), whereas those in both ST15 and multi-point groups were significantly lower than those 2 indexes in model group (P < 0.01). No significant differences were found between ST15 and multi-point groups in the diameter and area of AL. CONCLUSION: "Wuyi" (ST 15) can effectively suppress estrogenic hormones-induced hyperplasia of mammary glands and is thus a key acupoint for treatment of cyclomastopathy.