癫痫细胞悬液对海马干细胞分化的影响

目的 观察癫痫大鼠海马细胞悬液对海马干细胞分化的影响.方法 实验分为对照组、谷氨酸组(包括5 μmol/mL、25 μmol/mL2个亚组)、正常大鼠海马细胞悬液组(包括20μg/mL、40μg/mL 2个亚组)及癫痫细胞悬液组(包括20 μg/mL、40 μg/mL 2个亚组),分别向接种了海马干细胞神经球的96孔培养板内加入培养基、相应浓度谷氨酸、正常大鼠海马细胞悬液及癫痫细胞悬液,应用MTT法比较4组在分化第1、7、10、14天之间海马干细胞分化活力.结果随着分化时间的延长,各组MTT值均呈增加趋势.各浓度谷氨酸组及正常海马细胞悬液组细胞活力较对照组下降,而癫痫细胞悬液组的细胞活力较对照组轻微增加.但差异没有统计学意义(p>05).相同浓度的癫痫细胞悬液组与正常海马细胞悬液组之间 MTT值差异没有统计学意义(p>0.05).结论 癫痫细胞悬液未对海马干细胞的分化产生影响.

Effect of suspended cells from epileptic rats on differentiation of hippocampal stem cells

Objective To investigate the effect of suspended cells from the hippocampus of epileptic rats on the differentiation of hippocampal stem cells.Methods Eight groups, including control group,glutamic acid groups (5 and 25 μmol/mL groups),normal hippocampal cells and epileptic cells groups (20 and 40 μmol/mL groups,respectively) were chosen in the experiment.The suspended cells were extracted from the hippocampus of epileptic rats and normal rats,and corresponding dose of them were added into the epileptic cells groups and normal hippocampal cells groups,respectively.Five μmol/mL and 25 μmol/mL glutamic acid were added into the glutamic acid groups,respectively.The vitality of differentiated stem cells was detected and compared with MTT method.Results MTT value (the vitality of differentiated stem cells) was increased following the increased differentiation time.Cell viability in the glutamic acid groups and normal hippocampal cells groups was slightly decreased as compared with that in the control group (P>0.05).The cell viability in the epileptic cells groups was slightly increased as compared with that in the control group (P>0.05).The MTT value in the epileptic cells group and the normal hippocampal cells group was not obviously different (P>0.05).Conclusion The suspended cells from the hippocampus of epileptic rats do not affect the differentiation of the hippocampal stem cells.