丹参注射液对实验性兔肺气肿阻抑作用的研究

目的:探讨丹参注射液局部用药对实验性兔肺气肿的阻抑作用。方法:36只兔随机分为肺气肿模型对照组(n=12)、α₁抗胰蛋白酶(α₁-AT)治疗组(n=12)、丹参治疗组(n=12)。胰蛋白酶气管内滴注建立兔肺气肿模型,治疗组第14天起气管内滴注药物,每周1次,连用4周。检测各用药前不同时期静脉血及肺泡灌洗液(BALF)中α₁-AT含量,观察各组不同时间肺组织形态学变化、检测肺泡平均内衬间隔值(MLIV)及单位面积肺泡数(NAPS)。结果:各组肺泡灌洗液中α₁-AT水平于第2周前无统计学差异(P>0.05),第3,4,5,6,8周肺气肿组α₁-AT水平较α₁-AT治疗组及丹参组明显下降(P<0.01)。血液中α₁-AT水平同一时期各组无统计学差异(P>0.05),不同时期α₁-AT水平有显著差异(P<0.01)。第6,8周肺气肿组肺气肿形态学形成,肺泡内衬间隔值及单位面积肺泡数与α₁-AT治疗组及丹参治疗组均有显著差异(P<0.01)。丹参及α₁-AT治疗组形态学统计无差异(P>0.05)。结论:丹参在兔肺气肿形成中气管内滴注可增加局部肺组织α₁-AT的浓度;丹参及α₁-AT早期局部应用均可阻抑实验性肺气肿的形成,两者无统计学差异。

Effect of Dansen injection on experimental emphysema in rabbits

OBJECTIVE: To observe the effect of Dansen injection on the experimental emphysema in rabbits. METHOD: Thirty-six rabbits were randomized into emphysema group (n = 12), Dansen injection treated group (n = 12) and alpha1-antitrypsin(alpha1-AT) treated group (n = 12). The animal model of emphysema was induced by intratracheal instillation of porcine pancreatic elastase. Dansen injection and alpha1-ATwere instilled intratracheal in two treated group after 14 days with porcine pancreatic elastase, respectively, once a week, to continue for four weeks. The level of alpha1-AT in serum and in bronchoalveolar lavage fluid (BALF) were analyzed in different times. The mean linear intercept value (MLIV) and the numbers of alveolar per square (NAPS) of all groups were compared after eight weeks with porcine pancreatic elastase. RESULT: The levels of alpha1-AT in BALF were significantly different between treated groups and emphysema group after two weeks treatment, alpha1-AT levels of treated groups were more increased than those of emphysema group (P < 0.01). The levels of alpha1-AT in serum were similar at same times in different groups (P > 0.05), but were great different in different times. The MLIV and the NAPS were significantly different from emphysema group to treated groups in sixth and eighth weeks (P < 0.01), there is no difference between dancen group and alpha1-AT group. CONCLUSION: The contents of alpha1-AT in local pulmonary tissue could be improved by Dansen injection through intratracheal instillation during the information of emphysema in rabbits. The effect of Dansen injection and alpha1-AT on preventing formation of emphysema is similar.