| 亲和层析法纯化蚯蚓胆碱酯酶 |
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| 引用本文： | 杜泽涵,董泗建. 亲和层析法纯化蚯蚓胆碱酯酶[J]. 军事医学科学院院刊, 1986, 0(1) |
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| 作者姓名： | 杜泽涵 董泗建 |
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| 作者单位： | 军事医学科学院基础医学研究所(杜泽涵)，军事医学科学院基础医学研究所(董泗建) |
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| 摘 要： | 将Sepharose 4B经溴化氰活化接上己二胺,再与乙酰胆碱酯酶的可逆性抑制剂(间-羧苯二甲基碘乙基季胺盐)偶联,作为亲和层析凝胶。蚯蚓(Eiseaia foetida)从养殖场买回后,用水冲净,室温饥饿2～4天。用5%硫酸铵在4℃制成匀浆,13,000g离心。上清液用36%硫酸铵沉淀及Sephadex G-200柱处理后,通过亲和层析柱纯化,胆碱酯酶活性提高了1,084倍。
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| 关 键 词： | 蚯蚓 胆碱酯酶 亲和层析 |
PURIFICATION OF CHOLINESTERASE OF EARTHWORM BY AFFINITY CHROMATOGRAPHY |
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| Du Zehan,Dong Sijian Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PURIFICATION OF CHOLINESTERASE OF EARTHWORM BY AFFINITY CHROMATOGRAPHY[J]. Bulletin of the Academy of Military Medical Sciences, 1986, 0(1) |
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| Authors: | Du Zehan Dong Sijian Institute of Basic Medical Sciences Academy of Military Medical Sciences |
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| Abstract: | |
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| Keywords: | earthworm cholinesterase affinity chromatography |
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