大鼠离体海马脑片NO变化与LTP产生的关系

目的 观察离体脑片突触传递长时程增强(LTP)中一氧化氮(NO)变化的特点。方法 采用胞外记录方法检测LTP产生和维持。原位杂交技术检测NOS mRNA表达变化。生化反应方法检测NO含量和NO合酶(NOS)活性变化。结果 条件刺激10min后，LTP产生，NO含量和NOS活性均显著升高。NOSmRNA表达也明显增加。条件刺激60min后，LTP稳定维持，但NO含量和NOS活性却恢复到条件刺激前水平。结论 NO的显著升高过程主要发生在LTP形成的早期阶段。

Relationship between NO changes and LTP production in rat hippocampus in vitro

Objective To observe the time course of the changes in nitric oxide (NO) content and the activity of NO synthase (NOS) during hippocampal long term potentiation (LTP) in vitro . Methods The production and maintenance of LTP were detected by using extracelluar electrophysiological recording. NO content and NOS activity were determined by biochemical reaction. The expression of NOS mRNA was detected by in situ hybridization. Results Conditioning stimulation for 10 min induced LTP production and significant increases in NO content, NOS activity and expression of NOS mRNA. However, at 60 min after conditioning stimulation, LTP remained stably but NO content and NOS activity returned to the pre conditioning stimulation level. Moreover, the NOS mRNA was overexpressed at the early stage of LTP production. Conclusion Significant NO changes may occur at the early stage of LTP formation.