吡格列酮和罗格列酮对脂多糖诱导大鼠血管平滑肌细胞增殖的影响

目的:观察胰岛素增敏剂——噻唑烷二酮类(TZD)药物吡格列酮和罗格列酮对脂多糖(LPS)诱导的血管平滑肌细胞(VSMCs)异常增殖的影响。方法:组织块贴壁法培养大鼠胸主动脉血管平滑肌细胞;LPS(0.1,1,10mg·L-1)干预血管平滑肌细胞不同时间(0,12,24,48,72h)后,分别用吡格列酮和罗格列酮与10mg·L-1LPS共同孵育72h,四甲基偶氮咗盐微量酶比色(MTT)法检测血管平滑肌细胞增殖。结果:10mg·L-1LPS孵育72h导致血管平滑肌细胞增殖程度最明显(P<0.01);吡格列酮和罗格列酮可明显抑制LPS诱导的血管平滑肌细胞增殖。结论:噻唑烷二酮类代表药吡格列酮和罗格列酮均对LPS诱导的血管平滑肌细胞异常增殖具有抑制作用,该类药物可能在治疗心血管疾病方面有较好的应用前景。

The Effect of Pioglitazone and Rosiglitazone on Rat Vascular Smooth Muscle Cell Proliferation Induced by Lipopolysaccharide

Objective： To investigate the effects of Thiazolidinediones（TZDs）-pioglitazone（pio）and rosiglitazone（Rosi） on rat thoracic aortic smooth muscle cells （TASMCs）proliferation induced by lipopolysaccharide （LPS）in vitro. Method： VSMCs of thoracic aorta of rats were primarily cultured by the method of tissue adherence. The cells of 3-10 passages were used in the experiment. MTT method was used to determine the vascular smooth muscle cell proliferation. Results： Cell proliferation was obviously increased by 10mg ·L^-1 LPS ;Pioglitazone and rosiglitazone inhibited the LPS-indueed cell proliferation of VSMCs obviously（P〈0. 05）. Conclusion： Pio and Rosi were able to inhibit the VSMCs proliferation induced by LPS has a good prospect for its wide application in the prevention and treatment of atherosclerosis.