羟基喜树碱对膀胱癌细胞自分泌的影响

目的 检测膀胱癌细胞自分泌因子并探讨羟基喜树碱对自分泌的影响。方法 采用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术，在无血清培养情况下检测膀胱癌Ｔ２４细胞株自分泌因子ＩＧＦ－１、ＩＧＦ－２、ＩＧＦ－１受体、白细胞介素（ＩＬ）－６受体、ＩＬ－６受体表达情况，并进一步用半定量ＲＴ－ＰＣＲ方法观察膀胱灌流药物羟基喜树碱分别在０．００５、０．０１０、０．０２０ｍｇ／Ｌ浓度时对前述指标的影响。结果 经羟基喜树碱（０．０１０ｍｇ／Ｌ）处理后，ＩＧＦ－１、ＩＧＦ－２、ＩＧＦ－１受体、ＩＬ－６、ＩＬ－６受体分别下降下４４．８％，５９．７％，３９．３％，５１．５％及４６．２％，同未痉基喜树碱处理组相比差异有显著性（Ｐ〈０．０５）。结论 膀胱癌细胞可自分泌ＩＬ－６、ＩＧＦ－１、ＩＧＦ－２等自分泌因子，羟基喜树碱对膀胱肿瘤自分泌表达的

Effect of 10-hydroxycamptothecim on autocrine expression of T24 urinary bladder cancer cells

Objective To determine autocrine expression of bladder cancer cells and explore the mechanism of hydroxycamptothecin (HPT)on autocrine expression. Methods IL 6,IL 6 receptors,IGF 1,IGF 2,IGF 1 receptors mRNAs of T24 bladder cancer cells in the condition of serum free culture were investigated by semiquantitative RT PCR after bladder cancer cells treated by HPT at the concentrations of 0.005, 0.010, 0.020 mg/L respectively. Results Compared to the HPT untreated bladder cancer cells group,the intrinsic mRNA expression of IGF 1,IGF 2,IGF 1 receptors,IL 6,IL 6 receptors was reduced approximately by 44.8%,59.7%,39.3%,51.5% and 46.2% respectively after treatment of 0.010 mg/L HPT( P <0.05). Conclusion The down regulation of HPT on autocrine expression of bladder cancer may contribute to its mechanisms for antitumor activity.