脑梗死患者血浆HDL亚类组成及含量研究

目的:分析脑梗死患者血浆高密度脂蛋白(HDL)亚类组成及含量。方法:采用双向电泳-免疫印迹检测法分析50例脑梗死患者和50例正常者HDL亚类的组成及含量。结果:与对照组比较,脑梗死患者血浆中preβ2-HDL和HDL2b含量显著降低(均P<0.01),小颗粒preβ1-HDL含量显著增加(P<0.01);患者血浆三酰甘油(TG)水平与preβ1-HDL、HDL3b、HDL2a和HDL2b含量呈显著负相关(均P<0.05),HDL-C、载脂蛋白AⅠ含量与preβ1-HDL、preβ2-HDL、HDL3c、HDL3b、HDL3a、HDL2a和HDL2b含量呈显著正相关(均P<0.05)。结论:脑梗死患者血浆大颗粒HDL含量明显降低,小颗粒HDL含量明显增加,提示脑梗死患者HDL代谢过程异常。

Distribution and concentration of high density lipoprotein subclasses in patients with brain infarction

Objective:To study the distribution and concentration of high density lipoprotein(HDL) subclasses in brain infarction patients. Method:Apolipoprotein concentrations of plasma HDL subspecies in 50 brain infarction patients and 50 normal controls were determined by two-dimensional gel electrophoresis associated with immunodection method. Result:Compared with normal controls,the concentration of preβ2-HDL and HDL2b were significantly lower,and small-sized preβ1-HDL was significantly higher in brain infarction patients(both P<0.01).Correlation between HDL subclasses and lipids in brain infarction showed that the levels of triglyceride(TG) were negatively correlated with preβ1-HDL,HDL3b,HDL2a and HDL2b(both P<0.05),the levels of apoAⅠ and HDL-C were positively correlated with preβ1-HDL,preβ2-HDL,HDL3c,HDL3b,HDL3a,HDL2a,HDL2b(both P<0.05). Result:Compared with normal controls,the concentration of preβ2-HDL and HDL2b were significantly lower,and small-sized preβ1-HDL was significantly higher in brain infarction patients(both P<0.01).Correlation between HDL subclasses and lipids in brain infarction showed that the levels of triglyceride(TG) were negatively correlated with preβ1-HDL,HDL3b,HDL2a and HDL2b(both P<0.05),the levels of apoAⅠ and HDL-C were positively correlated with preβ1-HDL,preβ2-HDL,HDL3c,HDL3b,HDL3a,HDL2a,HDL2b(both P<0.05). Conclusion:The particle size of HDL shifted towards smaller sizes,which indicates that HDL metabolism might be abnormal in brain infarction patients.