承德地区莱姆病自然疫源性特征分析

目的了解承德地区莱姆病自然疫源地存在状况。方法于承德林区捕鼠、采蜱；巴伯-斯托纳-凯利培养基(BSK)分离蜱内病原体；PCR扩增蜱、鼠体内目的片段及序列测定；对林区居民及动物牛、羊进行血清抗体检测。结果全沟硬蜱为优势蜱种，从中PCR扩增出目的片段，基因序列同源性比较，提示此感染菌株与欧亚大陆常见的莱姆病螺旋体Borrelia afzelii种具有高度同源性。林区居民及动物牛、羊血清抗体检出阳性率较高，分别为13．3％，23．3％，30％。结论全沟硬蜱是承德地区的优势蜱种；并发现其感染有致病性莱姆病螺旋体Borrelia afzelii种。林区羊、牛为重要的贮存宿主。林区人群存在莱姆病螺旋体自然感染，可初步认定承德地区为莱姆病自然疫源地。

Analysis of zoonotic characters on lyme disease in forests of Chengde

Objective Preliminary investigation was conducted to make clear wehether Chengde forests areas was regarded as a natural foci of lyme disease.Methods Rodents and ticks were colected in Chengde forest areas.Some ticks were isolated and cultured Borrelia burgdorferi in BSK medium.Some ticks and rodents were amplified to the fragment of the expected by PCR.Venous blood was drawn from cattle and sheep and human in forest areas.IFA was conducted to test the titer of antibody of the sampled animals.Results I.persulcatus was the most dominant in the ticks collected.One strais I.persulcatus was amplified fragment of the expected size.Through the transformation gene clone and sequence analysized,the sequence amplified were blasted in GeneBank and high homologous to Borrelia afzelli,and it should be regarded as Borrelia afzelli species.The positive rate of residents and cattle and sheep serum antibody was 13.3%,23.3%,30% respectively.Conclusion I.persulcatus might be a dominant vector tick species while Lyme spirochete is detected from it.Cattle and sheep in forest areas were important reservoir hosts of lyme disease.The residents in forest areas also had been found infected with thelyme disease spirochetes.The results showed that chengde region was very important natural foci of lume disease.