Experimental–Computational Evaluation of Human Bone Marrow Stromal Cell Spreading on Trabecular Bone Structures |
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Authors: | B G Sengers C P Please M Taylor and R O C Oreffo |
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Institution: | (1) Bone & Joint Research Group, Institute of Developmental Sciences, University of Southampton, Southampton General Hospital, Mailpoint 887, Tremona Road, Southampton, SO16 6YD, UK;(2) School of Mathematics, University of Southampton, Highfield, Southampton, SO17 1BJ, UK;(3) School of Engineering Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK |
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Abstract: | The clinical application of macro-porous scaffolds for bone regeneration is significantly affected by the problem of insufficient
cell colonization. Given the wide variety of different scaffold structures used for tissue engineering it is essential to
derive relationships for cell colonization independent of scaffold architecture. To study cell population spreading on 3D
structures decoupled from nutrient limitations, an in vitro culture system was developed consisting of thin slices of human trabecular bone seeded with Human Bone Marrow Stromal Cells,
combined with dedicated μCT imaging and computational modeling of cell population spreading. Only the first phase of in vitro scaffold colonization was addressed, in which cells migrate and proliferate up to the stage when the surface of the bone
is covered as a monolayer, a critical prerequisite for further tissue formation. The results confirm the model’s ability to
represent experimentally observed cell population spreading. The key advantage of the computational model was that by incorporating
complex 3D structure, cell behavior can be characterized quantitatively in terms of intrinsic migration parameters, which
could potentially be used for predictions on different macro-porous scaffolds subject to additional experimental validation.
This type of modeling will prove useful in predicting cell colonization and improving strategies for skeletal tissue engineering. |
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Keywords: | Skeletal tissue engineering Cell migration Scaffold architecture Computational modeling |
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