a Faculty of Pharmacy, Institute of Analytical Chemistry, Belgrade, Yugoslavia
b Institute of Bosnalijek Company, Sarajevo, Yugoslavia
c Laboratory of Organic Chemistry and Biochemistry, Faculty of Science, Zagreb, Yugoslavia
Abstract:
Pralidoxime chloride (PAM-2Cl) has been determined spectrophotometrically in Britton—Robinson buffer solution at pH = 6.45; the method is based on measurement of the absorbance of the Pd(II)-pralidoxime complex at 327 nm. Studies of the composition of the complex by Job's continuous variation method, the molar ratio method and Bent—French's method yielded a Pd(II):pralidoxime ratio of 1:1. The conditional stability constant (K′) of the complex at the optimum pH of 6.45 and an ionic strength (μ) of 0.3 M was found to be 105.2. The molar absorptivity was 1.05 × 104 1 mol?1 cm?1. Beer's law was obeyed at concentrations up to 60 μM. The detection limit was 0.55 μg ml?1. The relative standard deviation (N = 10) was 0.28–1.03%. The method was accurate and sensitive for the analysis of PAM-2Cl in water and tablets.