再生障碍性贫血患者环孢素A血药浓度测定方法的比较

目的：寻找在监测再生障碍性贫血（再障）患者环孢素Ａ（ＣｓＡ）浓度时高效液相色谱（ＨＰＬＣ）法和多克隆荧光偏振免疫（ＰＦＰＩＡ）法两者之间的差异和相关性。方法：建立一种特异性测定再障患者血清服药后全血中ＣｓＡ原药的ＨＰＬＣ法，并与非特异性测定ＣｓＡ原药及其代谢物总浓度的Ｐ〈０．０１，经相关与回归分析呈线性关系，方程为：ＹＦＦＰＬＡ＝２．４７ＸＨＰＬＣ＋６９．５２，ｒ＝０．９１２４，ｎ＝３８，（Ｐ〈０

The comparison of HPLC and polyclonal FPIA determination of cyclosporine in aplastic anemia patient

AIM : To find the difference and comparison between HPLC and polyclonal flu-o-rescence polarization immunoassay (PFPIA) which determined cyclosporine A(CsA) in aplastic anemia (AA) patients. METHODS: A specific HPLC method for determination of whole blood concentration of parent CsA in AA patients receiving CsA was established and compared with nonspecificity PFPIA for determination of the summation of parent CsA and metabolites. RESULTS:Both determination value was tested by t-test for dependent sample (P<0. 01). Both determination value was analysed by correlation and regression. HPLC and PFPIA methods had a line relation. The curve equation was Y_(PFPIA) = 2. 47X_(HPLC) 69. 52, r = 0.912 4,n = 38,(P<0. 01). CONCLUSION:It is clear that the good method for determintion of whole blood concentration of CsA is both the summation of CsA_(parent metabolites) and CsA_(parent), Then the ratio was calculated. The variety of the ratio CsA_(parent metabolites)/CsA_(parent) shows that the patient's liver was abnormal or the patient had administered enzyme inductor or enzyme inhibitor,in order to adjust projects.