Deoxyribonucleic acid synthesis in cells replicating polyoma virus |
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| Authors: | R Sheinin |
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| Affiliation: | 1. Ontario Cancer Institute, University of Toronto, Toronto, Canada;2. Department of Microbiology, University of Toronto, Toronto, Canada;1. Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague 2, Czech Republic;3. Institute of Organic Chemistry and Biochemistry of the CAS, Flemingovo náměstí 542/2, 166 10 Prague 6, Czech Republic;1. Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, BSRB 3043, Ann Arbor, MI 48109, USA;2. Biomedical Research Core Facilities, University of Michigan Medical School, 109 Zina Pitcher Place, BSRB, Ann Arbor, MI 48109, USA;1. Department of Dermatology, University of Texas Medical School at Houston, TX 77030, United States;2. Baylor College of Medicine, Houston, TX 77030, United States |
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| Abstract: | The procedure for separation of polyoma DNA and mouse embryo cell DNA by chromatography on MAK columns (Dulbecco et al., 1965) has been extended. Native mouse embryo DNA was found to elute from MAK columns at concentrations of NaCl between 0.55 and 0.65 M, whereas heat-denatured mouse embryo DNA was not eluted even with 0.8 M NaCl. Heat-treated polyoma DNA was eluted from MAK columns at the same concentration of NaCl (0.53–0.57 M) which eluted native virus DNA.Using these properties of cell and viral DNA, the nature of the DNA synthesized in mouse embryo cells replicating polyoma virus has been examined. In exponentially growing cells, in which nearly all infected cells make virus, the DNA synthesized up to 12 hours after infection (i.e., during eclipse) was all cell DNA. After 12 hours the synthesis of cell DNA decreased to reach zero at about 18–20 hours after infection. Viral DNA synthesis began at about 12 hours after infection and increased progressively so that by 18–20 hours after infection all the DNA synthesized in the infected cultures was viral DNA. |
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