VEGF治疗退变性膝关节炎的动物实验研究

目的通过观察血管内皮生长因子（VEGF）对动物膝骨关节炎（OA）软骨的影响,探讨OA的发病机制及VEGF不同给药方式对其治疗作用。方法20只新西兰大白兔随机分为4组：正常对照组（A组）,模型对照组（B组）,VEGF膝关节腔注射组（C组）,VEGF腹腔注射组（D组）。手术建立OA模型,第8天开始D组腹腔注射VEGF每天1次,C组关节腔每7d注射VEGF1次,给药12周后处死动物,切取胫骨平台关节软骨大体观察,行苏木精-伊红染色及甲苯胺蓝染色检查,检测血清、关节液及滑膜中超氧化物歧化酶（SOD）、丙二醛（MDA）和一氧化氮（NO）。结果A组动物模型关节软骨正常;B、D两组动物模型关节软骨缺损部分修复;C组动物模型关节软骨缺损完全修复。与B组比较,C组关节软骨形态学上明显改善,血清及关节液中SOD明显升高（F=11.43,P〈0.01）,NO、MDA均明显降低（F=16.23,P〈0.01）,而D组则未见明显改善。结论VEGF能有效防治实验性OA;关节腔注射VEGF治疗OA的疗效明显优于腹腔注射。

EXPERIMENTAL STUDY OF THE EFFECT OF VASCULAR ENDOTHELIAL GROWTH FACTORS ON DEGENERITIVE OSTEOARTRITIS OF KNEE JOINTS

Objective To study the pathogenesis of osteoarthritis （OA） by investigating the effect of vascular endothelial growth factors （VEGF） on experimental OA in rabbits. Methods Twenty New Zealand rabbits were equally randomized to normal control （group A）, model control （group B）, VEGF joint-cavity-injection （group C） and VEGF-peritoneat injection （group D）. An OA model was established. Starting from the eighth day, intra-peritoneal injection of VEGF was given to rabbits in group B, once a day; intra articularl injection of VEGF to group C, once very seven days. The rabbits were sacrificed after 12 weeks of medication. Gross and histologic observation of articular cartilage was carried out. Levels of MDA, NO, and SOD in serum and synovial fluid were detected. Results In group A, the articular cartilage was normal; in groups A and D, the defect of articular cartilage was partly repaired; in group C, the defect was fully recovered. Compared with group B, the morphology of articular cartilage in group C significantly improved, SOD increased both in serum and synovial fluid （F= 11.43, P〈0.01）, and both the levels of MDA and NO decreased （F= 16.23,P〈0.01）, no obvious improvement was seen in group D. Conclusion VEGF can effec tively prevent and treat experimental OA, intraarticular injection is better than intraperitoneal injection.