HBV DNA与其五项检测指标关系及意义

目的探讨乙型肝炎病毒（HBV）标志物定量检测在慢性乙型肝炎诊治中的临床意义。方法选择住院慢性乙型肝炎病人70例，应用电化学发光法测定入院时第一份血清HBV相关抗原、抗体的含量，应用聚合酶链反应法测定HBV DNA含量，同时测定血清丙氨酸氨基转移酶（ALT）水平。结果慢性乙型肝炎病人HBV DNA与HBsAg和HBeAb呈负相关关系（r=-0．494、-0．536，P〈0．01），与HBeAg呈正相关关系（r=0．586，P〈0．01）；HBeAg阳性组HBV DNA与HBsAg和HBeAb呈负相关关系（r=-0．555、-0．568，P〈0．01），与HBeAg和HBcAb呈正相关关系（r=0．608、0．569，P〈0．01）；HBeAg阴性组HBV DNA与HBV抗原抗体指标不存在相关关系（P〉0．05）。慢性乙型肝炎病人ALT与HBV DNA及HBV抗原抗体指标均不存在相关关系（P〉0．05）。结论HBeAg、HBcAb、HBsAg定量检测是反映HBV复制的指标之一，HBsAg变化可能与HBV的生物学行为有关，无证据支持以乙型肝炎标志物来判断肝功能受损的程度。

THE RELATIONSHIP BETWEEN HBV DNA AND FIVE-ITEM QUANTITATIVE DETECTION AND ITS CLINICAL SIGNIFICANCE

Objective To assess the clinical significance of quantitative detection of HBV marker in the diagnosis and therapy of chronic hepatitis B （CHB）. Methods The first blood sample of 70 CHB patients on admission was detected for the contents of HBV＇s antibody and antigen by ECLIA, which were then determined for HBV DNA and ALT by PCR. Results HBV DNA in CHB showed negative correlation with HBsAg and HBeAb （r = - 0. 494, - 0. 536 ; P〈 0. 01） ; but positive with HBeAg （r=0. 586,P〈0.01）. In HBeAg-positive group, HBV DNA showed negative correlation with HBsAg and HBeAb （r= -0. 555-0. 568;P〈0.01）, and positive with HBeAg and HBcAb （r=0. 608,0. 569;P〈0.01）. In HBeAg-negative group, no associations were noted between HBV DNA and above HBV markers （P〉0.05）. The ALT in CHB patients did not show any correlativity with ALT and HBV markers （P〉0. 05）. Conclusion The quantitative detection of HBeAg, HBcAb and HBsAg is one of the parameters reflecting HBV duplication; the changes of HBsAg are probably related to biological behaviors of HBV. There is no evidence to support that the degree of liver function damage can be judged by HBV markers.