柔红霉素对大鼠心肌肌钙蛋白T及肌酸激酶水平影响

目的通过观察柔红霉素对大鼠血浆心肌肌钙蛋白T（cTnT）及肌酸激酶同工酶（CK-MB）水平的影响,探讨cTnT早期诊断柔红霉素心肌损伤的价值。方法将20只Wistar大鼠随机分为对照组和实验组,每组10只。对照组给予生理盐水1.0mL/kg尾静脉注射,实验组给予柔红霉素2.0mg/kg尾静脉注射,两组均每3d注射一次,共6次。于给药第0、6、12、18天,分别测定大鼠血浆cTnT及CK-MB的含量。结果给药前后对照组大鼠血浆cTnT及CK-MB浓度均无明显改变（F=2.69、1.02,P〉0.05）,而实验组大鼠在给药后血浆cTnT含量较给药前显著增加（F=44.20,P〈0.01）,CK-MB在给药前后差异无统计学意义（F=0.24,P〉0.05）。结论应用柔红霉素后大鼠早期血浆cTnT含量升高较CK-MB更为敏感。

THE EFFECTS OF DAUNORUBICIN ON TROPONIN AND CREATINE KINASE IN RATS

Objective To assess the value of plasma cardiac troponin T （cTnT） in early diagnosis of daunoruhicin （DNR）-induced myocardial damage by observation the effects of DNR on plasma creatine kinase isoenzyme （CK-MB） and cTnT in rats. Methods Twenty Wistar rats were evenly randomized to experimental and control groups. DNR, 2.0 mg/kg, was given to experimental group; and normal saline, 1.0 mL/kg, was given to control group, both groups were administered by tail vein injection, once every three days for six injections. The levels of plasma CK-MB and cTnT were measured at 0, 6, 12 and 18 day. Results The levels of plasma cTnT and CK-MB in control group were not much changed before and after medication （F=2.69, 1.02;P〉0. 05）. In experimental group, the cTnT level dramatically increased as opposed to before medication （F= 44.20, P〈 0.01）, but the difference of CK-MB before and after medication did not show statistical significance （F=0.24,P〉0.05）. Conclusion After injection of DNR in rats, the early rise of plasma cTnT is more sensitive than CK-MB.