CTGF在单侧输尿管梗阻大鼠肾组织中的表达以及活性维生素D_3干预治疗的研究

目的：观察单侧输尿管梗阻大鼠肾脏组织中CTGF的表达以及活性维生素D3（1,25-（OH）2D3）对其表达的影响。探讨1,25-（OH）2D3对大鼠肾间质纤维化的调节作用。方法：随机采用体重180~220g的清洁级Wistar大鼠,雄性,36只,随机分为假手术组（n=12）、UUO模型组（n=12）、活性维生素D3组（n=12）。造膜后活性维生素D3组给予罗盖全（骨化三醇）灌胃（3ng/100g）,假手术组及UUO模型组给予等量生理盐水灌胃。给药后第14天,28天分批（n=6）处死,取造膜侧肾脏。用HE染色及Masson染色方法观察肾脏组织纤维化程度,用免疫组化法测定结缔组织生长因子（CTGF）在各组大鼠造膜侧肾脏的表达情况。结果：与假手术组比较,UUO模型组大鼠的肾脏组织中CTGF的表达有显著增高（P〈0.05）,而在活性维生素D3治疗组中,CTGF的表达低于UUO模型组（P〈0.05）,肾间质纤维化程度也明显小于模型组（P〈0.01）。结论：CTGF在UUO大鼠肾脏中表达明显增高,1,25（OH）2D3能有效抑制UUO大鼠肾脏组织CTGF的表达,从而减轻UUO大鼠肾脏组织纤维化程度。

The expression of CTGF in rat UUO and the intervention study of 1,25(OH)2D3

Objective:To observe the expression of connective tissue growth factor(CTGF) in rat kidneys with unilateral ureteral obstruction,and explore the role of 1,25-(OH)2D3 on Renal interstitial fibrosis.Methods:Thirty-three Wistar rats were randomly divided into sham-operation group(n=12),UUO model group(model group,n=12) and Treatment with 1,25-(OH2D3 group(n=12).Treatment with 1,25-OH)2D3 group was treated with Rocaltrol(calcitriol)(3ng/100g) by the gastric perfusion.Sham-operation group and UUO model group were given the same amount of normal saline.Rats were sacrificed 14 and 28 days after operation(after model establishment for model group)(n=6),and the renal tissues of the operated side(obstructed side for model group)were obtained.The renal fibrosis was observed with HE staining method and Masson staining method.The expression of CTGF in rat kidney of each group was determined by immunohistochemical method.Results:Compared with the sham group,the expression of CTGF in the kidney tissue in UUO model group rats was significantly higher(P<0.05),while the expression of CTGF in the treatment group is lower than the UUO model group(P<0.05),renal interstitial fibrosisdegree significantly less than model group(P<0.01).Conclusion:CTGF was significantly increased in UUO rat kidney.1,25(OH)2D3 can effectively inhibit the expression of CTGF in UUO rat kidneys,thereby reduce the fibrosis of UUO rat kidney.