酸味中药复方对2型糖尿病大鼠主动脉AGEs含量及受体基因表达的影响

目的 观察酸味中药复方对2型糖尿病(T2DM)大鼠主动脉晚期糖基化终产物(AGEs)含量及受体(RAGE)基因表达的影响，探讨其防治糖尿病大血管病变的机制。

Effect of Compound Chinese Sour Taste Herbs on Advanced Glycation End Products Content and Gene Expression of Their Receptors in Aorta of Rats with Type 2 Diabetes Mellitus

Objective To investigate the possible mechanism of compound Chinese sour taste herbs （CS） in preventing and ameliorating diabetic macroangiopathy by analyzing the effects of CS on the deposition of advanced glycation end products （AGEs） and gene expression of receptor for advanced glycation end products （RAGE） in the aorta tissue of rats with type 2 diabetes mellitus （T2DM）. Methods Rat model of T2DM was established by peritoneal injection of streptozotocin （STZ） and high caloric diet feeding. Experimental SD rats were divided into the normal group, the model group, the aminoguanidine （AG） group, and the CS group. At the end of the 8th and 12th week, the fasting blood glucose （FBG） was measured by glucose oxidase method; content of AGEs and collagen in aorta detected by fluorescent method and gene expression of RAGE in aorta determined by Real-time PCR method. Results FBG, AGEs＇and collagen contents and RAGE expression in aorta of model rats were all higher than those in the normal control group （ P 〈 0.05）, while all these indices were lower in the CS group than in the model group （P 〈0.05）. Conclusion CS could realize the goal for preventing and ameliorating diabetic macroangiopathy by way of suppressing the production of AGEs and down-regulating the gene expression of RAGE in aorta of T2DM rats.