转染外源性PTEN基因人胃癌细胞生长情况及其对化疗药物敏感性的观察

目的观察转染外源性PTEN基因人胃癌细胞的生长情况及其对化疗药物敏感性的影响。方法人胃癌BGC823细胞转染PEAK8空载质粒和PEAK8-PTEN质粒，稳定表达后予足叶乙甙和阿霉素干预；RT—PCR法检测PTENmRNA表达，MTT法检测细胞生长活力，流式细胞术分析细胞周期。结果转染PEAK8-PTEN质粒的BGC823细胞PTEN mRNA强表达，明显高于转染空载质粒和未转染细胞；PTEN转染后肿瘤细胞存活率下降（P〈0．05），联合化疗药细胞存活率更低（P〈0．01）；转染PTEN加两种化疗药处理后均出现G2／M为0，细胞阻断于S期。结论转染PTEN基因的人胃癌BGC823细胞生长受抑制，对化疗药敏感性增加。

Growth and susceptibility to chemical therapy drug of gastric carcinoma cell transfected oxogenous PTEN gene

Objective To observe the growth and susceptibility to chemical therapy drug of gastric carcinoma cell transfected exogenous PTEN gene. Methods PEAK8 and PEAK8-PTEN plasmid were transferred into gastric carcinoma cell line BGC823 . The stable expression cell line was interfered by etoposide and doxorubicin hydrochloride. The expression of target genes was detected by RT-PCR. Cell viability was determined by MTT assay. Cell cycle analysis was determined by flow cytometry. Results The expression of PTEN was verified in group transfected with PEAK8-PTEN plasmid, but not in PEAK8 group and the control group . The groups transfected with PTEN had much lower cell viability than corresponding ones（ P 〈 0.05 ） , especially in PEAK8-PTEN group interfered by chemical drug meanwhile （P 〈 0.01 ） . PTEN genc transfection could block cell cycle before S stage. Conclusion PTEN gene transfection could suppress the growth of gastric carcinoma cell line BGC823 and enhance its susceptibility to chemical therapy drug in vitn.