乙肝患者血清病毒标志物与HBV-DNA的检测结果分析

目的:探讨乙型肝炎患者体内乙肝病毒(HBV)标志物与乙肝病毒脱氧核糖核酸(HBV-DNA)的关系及其临床意义。方法:采用酶联免疫吸附试验(ELISA)检测HBV血清标志物,并对212份HBV标志物阳性标本采用荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA。结果:Ⅰ组(HBsAg、HBeAg、抗-HBc三项阳性)、Ⅱ组(HBsAg、抗-HBe、抗-HBc三项阳性)、Ⅲ组(HBsAg、抗-HBc阳性)血清中HBV-DNA阳性率分别为97.17%、47.13%、47.37%;Ⅰ组血清中HBV-DNA阳性率显著高于Ⅱ组与Ⅲ组血清中HBV-DNA阳性率(P<0.01)。结论:患者血清HBV-DNA的阳性率与HBV血清标志物的存在状态相关。检测乙肝病毒,采用FQ-PCR法检测HBV-DNA能更准确、直接地反映体内病毒复制情况。

Analysis on Detection of Serum HBV Markers and HBV-DNA in Hepatitis B Patients

Objective: To investigate the relationship and clinical significance between serum HBV markers and HBV-DNA in hepatitis B patients.Methods: Serum HBV markers were detected by ELISA and serum HBV-DNA was detected by fluorescence quantitative polymerase chain reaction(FQ-PCR) technology in the 212 patients whose serum HBV markers were positive.Results: The positive rates of serum HBV-DNA were 97.17%,47.13% and 47.37% in the group Ⅰ,Ⅱ and Ⅲ group respectively.The positive rate of serum HBV-DNA in the group Ⅰwas significantly higher than the one in the group Ⅱ or in the group Ⅲ.Conclusion: The positive rate of serum HBV-DNA was related to the mode of serum HBV markers.The result of serum HBV-DNA that was detected by FQ-PCR can reflect correctly and directly the replication of hepatitis B virus.