| 钙蛋白酶抑制剂对缺血-再灌注心肌肌浆网钙泵功能及其蛋白水平的影响 |
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| 引用本文： | 王金桂,祝宝华,于晓頔,陈开祥,奚群英,刘晨. 钙蛋白酶抑制剂对缺血-再灌注心肌肌浆网钙泵功能及其蛋白水平的影响[J]. 江苏医药, 2011, 37(2) |
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| 作者姓名： | 王金桂 祝宝华 于晓頔 陈开祥 奚群英 刘晨 |
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| 作者单位： | 1. 无锡市中医医院ICU,214037
2. 扬州市,东南大学附属扬州医院心血管病研究所 |
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| 基金项目： | 江苏省135工程医学重点人才课题资助基金项目(RC2002016) |
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| 摘 要： | 目的研究钙蛋白酶抑制剂ALLN对缺血-再灌注(I-R)大鼠心肌肌浆网钙泵(SERCA)功能的影响。方法 24只雄性SD大鼠随机分为正常灌注组(对照组)、I-R组、ALLN+I-R组和二甲亚砜+I-R组(DMSO+I-R组),分别检测再灌注15min时冠状动脉流出量(CF)和冠脉流出液中乳酸脱氢酶(LDH)漏出量;荧光分光光度计测定荧光强度来反映钙蛋白酶活性;分别采用改良p-NPP法和Millipore过滤技术测定SERCA的活性和ATP依赖的SERCA45Ca2+摄取率,免疫印迹法检测心肌细胞SERCA蛋白表达水平。结果与I-R组相比,ALLN+I-R组CF、SERCA蛋白表达水平、SERCA的活性和45Ca2+摄取率显著增高(P<0.01),而LDH漏出量和钙蛋白酶活性显著降低(P<0.01)。结论 ALLN对离体大鼠心脏I-R损伤有保护作用,与其抑制钙蛋白酶活性,一定程度减轻I-R损伤对SERCA功能及蛋白表达水平的影响有关。
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| 关 键 词： | 缺血-再灌注损伤 钙蛋白酶 肌浆网钙泵 |
Effects of calpain inhibitor on function and expression of sarcoplastic reticulum Ca2+-ATPase in isolated ischemia-reperfusion hearts of rats |
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| WANG Jingui,ZHU Baohua,YU Xiaodi,et al.. Effects of calpain inhibitor on function and expression of sarcoplastic reticulum Ca2+-ATPase in isolated ischemia-reperfusion hearts of rats[J]. Jiangsu Medical Journal, 2011, 37(2) |
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| Authors: | WANG Jingui ZHU Baohua YU Xiaodi et al. |
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| Affiliation: | WANG Jingui,ZHU Baohua,YU Xiaodi,et al.Department of Critical Care Medicine,Wuxi Hospital of Traditional Chinese Medicine,Wuxi 214037,CHINA |
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| Abstract: | Objective To investigate the protective effects of calpain inhibitor(ALLN) on sarcoplastic reticulum Ca2+-ATPase(SERCA) in isolated ischemia-reperfusion(I-R) hearts of rat.Methods Twenty-four male SD rats were randomly divided into 4 groups of control,I-R,ALLN+I-R,and DMSO+I-R with 6 rats each.At 15 min after reperfusion,coronary flux(CF) and LDH were examined,the activity of calpain was detected by fluorospectrophotometer,and SERCA activity and 45Ca2+ uptake were evaluated with modified p-NPP and Millipore... |
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| Keywords: | Ischemia-reperfusion injury Calpain Sarcoplastic reticulum Ca2 -ATPase |
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