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| 神经胶质瘤细胞株H MGA1基因沉默对吉西他滨化疗敏感性的影响 | |
| 引用本文： | 曹远东,黄培林,孙新臣,金志良,肖韡,马珺,邓雨霞.神经胶质瘤细胞株H MGA1基因沉默对吉西他滨化疗敏感性的影响[J].江苏医药,2011,37(7). |
| 作者姓名： | 曹远东 黄培林 孙新臣 金志良 肖韡 马珺 邓雨霞 |
| 作者单位： | 1. 东南大学医学院附属中大医院肿瘤科,南京市,210009 2. 东南大学医学院病理学与病理生理学系 3. 南京医科大学第一附属医院放疗科 |
| 基金项目： | 南京市医学科技发展项目(ZKX05030); 全军医学科学技术研究“十一五”计划项目(06MA111) |
| 摘 要： | 目的探讨神经胶质瘤细胞株SHG-44高迁移率族蛋白A1(HMGA1)基因表达下调后对吉西他滨化疗敏感性的影响。方法用HMGA1 siRNA慢病毒载体及阴性siRNA慢病毒载体分别转染SHG-44细胞。稳定转染HMGA1 siRNA的细胞为阳性组、稳定转染不含HMGA1的细胞为阴性组,未转染的细胞为对照组。RT-PCR和Western blot检测HMGA1 mRNA和蛋白的表达,MTT法和克隆集落形成实验检测吉西他滨对细胞生长、增殖的影响,流式细胞术检测细胞凋亡率。结果阳性组HMGA1 mRNA和蛋白的表达水平明显低于阴性组和对照组(P<0.05)。经吉西他滨处理后,MTT表明阳性组细胞生长抑制率显著高于阴性组,阳性组IC50为(0.279±0.013)μg/ml,明显低于阴性组的(0.746±0.020)μg/ml(P<0.05)。克隆形成实验表明阳性组克隆形成率显著低于阴性组(P<0.05),而细胞凋亡率显著高于阴性组(P<0.05)。结论神经胶质瘤细胞SHG-44中HMGA1基因沉默后,显著增强SHG-44细胞对吉西他滨的化疗敏感性。 |
| 关 键 词： | 神经胶质瘤 高迁移率族蛋白A1 吉西他滨 |
Effect of HMGA1 gene silencing in glioma cell lines on gemcitabine chemotherapy sensitivity |
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| CAO Yu,ong,HUANG Peilin,SUN Xinchen,et al..Effect of HMGA1 gene silencing in glioma cell lines on gemcitabine chemotherapy sensitivity[J].Jiangsu Medical Journal,2011,37(7). | |
| Authors: | CAO Yu ong HUANG Peilin SUN Xinchen |
| Institution: | CAO Yuandong,HUANG Peilin,SUN Xinchen,et al.Department of Oncology,Affiliated Zhongda Hospital,Southeast University,Nanjing 210009,CHINA |
| Abstract: | Objective To evaluate the effect of down expression of HMGA1 gene in glioma cell lines SHG-44 on gemcitabine chemotherapy sensitivity.Methods Lentiviral vectors with HMGA1 siRNA and negative siRNA lentiviral vectors were transfected into SHG-44 cells.Stably transfected HMGA1siRNA cells were taken as positive group,stably transfected cells without HMGA1 as negative group,while the non-transfected cells as controls.The expression of HMGA1 was determined by RT-PCR and Western blot.MTT method and clonogenic ass... |
| Keywords: | Glioma High mobility group A1 Gemcitabine |
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