| 与肌源性干细胞共培养改善大鼠胰岛活性 |
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| 引用本文： | 王欣燕,刘畅,梅晰凡,姜丁文,王筠.与肌源性干细胞共培养改善大鼠胰岛活性[J].江苏医药,2011,37(5). |
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| 作者姓名： | 王欣燕 刘畅 梅晰凡 姜丁文 王筠 |
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| 作者单位： | 1. 辽宁医学院第一附属医院内分泌病科,辽宁省锦州市,121000
2. 辽宁医学院第一附属医院骨科,辽宁省锦州市,121000 |
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| 基金项目： | 辽宁省教育厅创新团队项目(2008T116) |
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| 摘 要： | 目的研究体外共培养模型中肌源性干细胞(MDSCs)改善大鼠胰岛活性的作用。方法提取新生大鼠MDSCs原代细胞,差速贴壁培养后行结蛋白免疫细胞化学鉴定,取第4代MDSCs接种在transwell底板。提取大鼠胰岛后,双硫腙染色鉴定,胰岛在高糖环境中处理24 h后放入tran-swell板上层与MDSCs共培养。在培养的第1、3、6天分别用吖啶橙/溴化乙锭(AO/EB)双荧光染色胰岛,RT-PCR检测胰岛的Bcl-2/Bax基因表达来评价胰岛凋亡,其结果与单独胰岛培养组比较。结果与单独胰岛培养相比,共培养组第3、6天AO/EB染色见胰岛细胞明显增多,Bcl-2明显升高,而Bax明显降低(P<0.05、P<0.01)。结论大鼠胰岛与MDSCs共培养延长大鼠胰岛存活时间,改善胰岛活性。
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| 关 键 词： | 肌源性干细胞 胰岛 |
Co-culture in vitro with muscle-derived stem cells improves the viability of pancreatic islets |
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| WANG Xinyan,LIU Chang,MEI Xifan,et al..Co-culture in vitro with muscle-derived stem cells improves the viability of pancreatic islets[J].Jiangsu Medical Journal,2011,37(5). |
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| Authors: | WANG Xinyan LIU Chang MEI Xifan |
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| Institution: | WANG Xinyan,LIU Chang,MEI Xifan,et al.Department of Endocrinology,First Affiliated Hospital,Liaoning Medical College,Jinzhou 121000,CHINA |
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| Abstract: | Objective To study the effect of co-culture in vitro with muscle-derived stem cells(MDSCs) on the function of the pancreatic islets in rat model.Methods MDSCs were extracted by mixed enzymatic digestion and purified by differential adherent culture,which were identified with Desmin immunocytochemical staining.MDSCs of the fourth generation were inoculated in the bottom room of transwell.Rat pancreatic islets were extracted and then identified by DTZ dyeing.After the islets had been brought up in high sugar ... |
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| Keywords: | Muscle-derived stem cells Pancreatic islets |
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