| 电针对脾虚证幼鼠海马区神经干细胞增殖和分化的影响 |
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| 引用本文： | 卓缘圆,杨卓欣,吴家满. 电针对脾虚证幼鼠海马区神经干细胞增殖和分化的影响[J]. 针刺研究, 2011, 36(5): 327-334 |
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| 作者姓名： | 卓缘圆 杨卓欣 吴家满 |
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| 作者单位： | 1. 深圳市中医院针灸科,深圳,518033
2. 深圳市龙岗中心医院康复科,深圳,518116 |
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| 基金项目： | 2007年广东省科技厅计划项目(73127) |
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| 摘 要： | 目的:通过观察电针对脾虚证幼鼠海马齿状回区神经干细胞增殖和分化的影响,探讨电针对中枢神经细胞损伤的保护和治疗作用。方法:4周龄SD雄性大鼠,随机分为正常组、模型组、电针组,每组各24只。3组再分别随机分为7、14、28和49 d等4个亚组,每亚组6只。用利血平腹腔注射联合大黄灌胃造脾虚证模型。造模结束后,电针组取双侧"足三里"和"三阴交"穴,每次电针20 min,每日1次,直至大鼠被处死。运用双重免疫组化法检测大鼠海马齿状回区5-溴脱氧尿苷(Brdu)、Brdu/巢蛋白(Nestin)、Brdu/神经胶质纤维酸性蛋白(GFAP)、Brdu/神经元特异性烯醇化酶(NSE)阳性细胞数。结果:与正常组同时相比较,Brdu、Brdu/GFAP、Brdu/NSE阳性细胞的表达在模型组7 d组和14 d组显著降低(P<0.05);Brdu、Brdu/Nestin、Brdu/GFAP阳性细胞的表达高峰出现在电针组14 d组,而Brdu/NSE阳性细胞表达的高峰出现在电针组28 d组,与同时相模型组比较差异具有统计学意义(P<0.05)。结论:脾虚证可导致神经干细胞增殖和分化能力降低。电针"足三里"三阴交"穴可促进脾虚证大鼠海马齿状回区神经干细胞的增殖,适当地促进增殖的神经干细胞向星形胶质细胞及神经元方向分化。
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| 关 键 词： | 电针 脾虚证 海马 神经干细胞 增殖 分化 |
Effect of electroacupuncture on differentiation and proliferation of hippocampal nerve stem cells in splenic asthenia pedo-rats |
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| ZHUO Yuan-yuan , YANG Zhuo-xin , WU Jia-man. Effect of electroacupuncture on differentiation and proliferation of hippocampal nerve stem cells in splenic asthenia pedo-rats[J]. Acupuncture research, 2011, 36(5): 327-334 |
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| Authors: | ZHUO Yuan-yuan YANG Zhuo-xin WU Jia-man |
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| Affiliation: | ZHUO Yuan-yuan,YANG Zhuo-xin,WU Jia-man1(Department of Acu-moxibustion,Shenzhen Municipal Hospital of Chinese Medicine,Shen-zhen 518033,China,1Rehabilitation Department of Longgang District Central Hospital of Shen-zhen,Shenzhen 518116) |
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| Abstract: | Objective To observe the effect of electroacupuncture(EA) on the differentiation and proliferation of nerve stem cells in the hippocampal dentate gyrus(DG) in splenic asthenia pedo-rats so as to study its central mechanism.Methods A total of 72 SD male rats were randomly assigned to normal control group(n=24),model group(n=24) and EA group(n=24) which were further divided into 7 d,14 d,28 d and 49 d time-points(n=6).Splenic asthenia model was established by intrape-ritoneal injection of reserpine and gavage... |
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| Keywords: | Electroacupuncture Splenic asthenia syndrome Hippocampus Nerve stem cell Proliferation Differentiation |
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