核因子кB与涎腺腺样囊性癌微血管密度、临床病理及预后的关系

目的 检测涎腺腺样囊性癌（ACCs）中核因子KB（NF-kB）p65的蛋白表达水平和微血管密度（MVD），并探讨二者与ACCs临床病理及预后的关系。方法用免疫组化法检测80例ACCs中NF-kBp65的表达，并以正常涎腺组织作对照；用CD34标记肿瘤血管内皮细胞计数肿瘤MVD，并对所有病例进行随访。结果NF-kB p65的表达与肿瘤MVD呈显著正相关（P〈0．01）。NF-kB p65和肿瘤MVD与ACCs的大小、TNM分期、组织学类型、侵犯血管和神经、复发及远处转移有关（P〈0．05）。NF-kB p65的表达、肿瘤MVD、肿瘤组织学类型及侵犯神经是影响ACCs预后的独立因素（P〈0．05）。结论ACCs中NF-kB p65的异常高表达与MVD呈正相关，且二者可视为影响ACCs预后的重要因素。

The relation of nuclear factor kappa B to angiogenesis and clinical outcomes in adenoid cystic carcinoma of salivary glands

OBJECTIVE: To evaluate the expression of nuclear factor kappa B (NF-kappaB) p65 in relation to angiogenesis microvessel density (MVD) and clinical outcomes in adenoid cystic carcinoma of salivary glands (ACCs). METHODS: Immunohistochemical staining method was used to quantify the protein expression levels of NF-kappaB p65 in 80 surgically resected salivary gland ACC and normal salivary tissues. In all cases of ACCs, microvessel density was evaluated by counting any CD(34)-reactive endothelial cell or endothelial-cell cluster. The patients with ACCs were followed up from 1992 to 2002. RESULTS: NF-kappaB p65 was detected in the cytoplasm of normal and ACC cells, but was only in the nucleus of tumor cells; while there was no NF-kappaB p65 nuclear staining in all the controls. The mean value of MVD was (47.07 +/- 13.44), which had significant correlations with NF-kappaB p65 expression (P < 0.01). In three histological types of salivary gland ACC, the expressions of NF-kappaB and MVD were significantly higher in solid type than in cribriform type and tubular type (P < 0.01). NF-kappaB p65 expression and MVD were significantly correlated to clinical stage, tumor size, perineural and vascular invasion, recurrence and metastasis (P < 0.05), but there were no correlations between those three factors and patient age, gender and tumor site (P > 0.05). Multivariate analysis showed NF-kappaB (P < 0.05) expression, MVD (P < 0.01), histotypes (P < 0.01), and perineural invasion (P < 0.05) had an independent prognostic impact on overall survival. CONCLUSIONS: The expression of NF-kappaB p65 was related to MVD, and the correlation between those two factors and clinico-pathological factors and prognosis of ACCs are significant.