灰树花多糖体外抑菌及抗氧化活性研究

目的 研究灰树花多糖的抑菌及抗氧化活性。方法 采用紫外分光光度法，以D-无水葡萄糖为对照品，测定灰树花提取物中多糖含量；采用纸片扩散法，以头孢哌酮为阳性对照，测量不同浓度灰树花多糖提取物金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑菌圈直径；采用DPPH法和T-AOC试剂盒法，以清除率为指标，研究灰树花多糖的抗氧化活性。结果 灰树花提取物中多糖含量为53.49%；灰树花提取物对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌均有一定的抑菌作用，抑菌圈直径分别为13，11，10 mm；灰树花多糖浓度为0.05~10 mg·mL^-1时，对DPPH自由基具有一定的清除能力，当浓度高于0.5 mg·mL^-1时，开始显示出总抗氧化能力。结论 灰树花多糖具有一定的抑菌和抗氧化能力。

Study on Antibacterial in Vitro and Antioxidant Activity of Grifola Frondosa Polysaccharide

OBJECTIVE To study the antibacterial effect and antioxidant activity of Grifola frondosa polysaccharide. METHODS UV spectrophotometry was used to determine the content of polysaccharide, D-anhydroglucose was used as a standard. The antibacterial effect produced by Kirby-Baue method with the bacteria of Staphylococcus aureus, Escherichia coli and Bacillus subtilis. Took the scavenging capacity as index to estimate the endogenous antioxidative activity of DPPH and T-AOC. RESULTS The total polysaccharides in Grifola frondosa extract was 53.82%. Grifola frondosa polysaccharide had a good antibacterial effect on Staphylococcus aureus, Escherichia coli and Bacillus subtilis, the diameter of antibacterial were 13, 11, 10 mm. With the concentration between 0.05~10 mg·mL^-1, Grifola frondosa polysaccharide had a good scavenging activity by DPPH, when the concentration above 0.5 mg·mL^-1, showed the total antioxidant activity. CONCLUSION The polysaccharides in Grifola frondosa has certain antibacterial effect and antioxidant activity.