Two new terpenoids from Reduning Injection |
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Authors: | Hai-bo Li Biao Yang Wen Ge Ze-yu Cao Liang Cao Yang Yu Zhen-zhong Wang Xin-sheng Yao Wei Xiao |
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Affiliation: | 1. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;2. State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;3. Zhejiang Pharmaceutical College, Ningbo 315000, China;4. Institute of Traditional Chinese Medicine & Natural Products, Guangdong Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China |
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Abstract: | ObjectiveTo study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning (RDN) Injection.MethodsIn this study, the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model. The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins, silica gel, ODS columns and reverse phase MPLC and HPLC repeatedly, and their structures were elucidated based on spectroscopic analysis (HR-ESI-MS, NMR, ECD) and chemical methods. Meanwhile, we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-α production in LPS stimulated RAW 264.7 macrophages.ResultsThe 95% ethanol eluate of RDN Injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection. A novel iridoid, named jasminoide A (1), and a new guaiane sesquiterpenoid, named (1R,7R,8S,10R)-7,8,11-trihydroxy-4-guaien-3-one (2), were isolated from Reduning injection, and compound 2 can inhibit TNF-α production with IC50 values of 72.24 µmol/L.ConclusionIn this study, two new terpenoids were isolated from Reduning Injection, and compound 2 showed inhibitory activity against LPS-activated TNF-α production in RAW 264.7 cells in vitro. |
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