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Effect of lead exposure on dopaminergic receptors in rat striatum and nucleus accumbens
Authors:S. Govoni   L. Lucchi   C. Missale   M. Memo   P.F. Spano  M. Trabucchi
Affiliation:1. Institute of Pharmacology and Pharmacognosy, University of Milan Italy;2. Department of Pharmacology and Therapeutics, University of Brescia Italy;3. Chair of Toxicology, 2nd University of Rome Italy;1. Departamento de Psicología y Sociología, Facultad de Ciencias Sociales y Humanas, Universidad de Zaragoza, Campus Ciudad Escolar, 44003 Teruel, Spain;2. Departamento de Psicología, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario CeiA3, La Cañada, 04120 Almería, Spain;3. Research in Neurobehavior and Health (NEUROLAB), and Research Center in Behavioral Assessment (CRAMC), Universitat Rovira i Virgili, Tarragona, Spain;4. Department of Molecular Pharmacology, Albert Einstein College of Medicine of Yeshiva University, Bronx, 10461 NY, USA;1. Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA;2. Department of Psychology, Auburn University, Auburn, AL, USA;1. College of Mechanical and Automotive, South China University of Technology, Guangzhou, 510640, China;2. Guangdong Provincial Key Laboratory of Technique and Equipment for Macromolecular Advanced Manufacturing, South China University of Technology, Guangzhou, 510640, China;3. SCNU Qingyuan Institute of Science and Technology Innovation Co., Ltd., Qingyuan, 511517, China;3. Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, Universitat Rovira i Virgili, Reus, Spain;4. Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona, Spain;1. Central Institute for Experimental Animals, Kawasaki-ku, Kawasaki 210-0821, Japan;2. Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan
Abstract:Haloperidol- and sulpiride-displaceable [3H]spiroperidol binding and the dopamine-inhibited adenylate cyclase were measured in rats chronically exposed to lead acetate. Haloperidol-displaceable [3H]spiroperidol binding was unmodified while sulpiride-displaceable binding was increased in striatum and decreased in nucleus accumbens. In addition, the decrease of sulpiride-displaceable binding in nucleus accumbens was paralleled by a reduced ability of bromocriptine to inhibit cAMP formation in presence of the D1 receptor antagonist SCH 23390. The results support the concept that in vivo lead treatment affects dopaminergic receptors and that the binding sites labelled by [3H]spiroperidol displaced by haloperidol may be different from those which recognize sulpiride.
Keywords:dopamine receptor   lead   striatum   nucleus accumbens
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