抗肿瘤坏死因子抗体和头孢噻甲羧肟对急性胆道感染肝损害的保护作用

在急性胆道感染大鼠模型上检测血浆肿瘤坏死因子（ＴＮＦ）、内毒素（ＥＴ）水平及血清谷丙转氨酶（ＡＬＴ）活性；观察肝组织结构和超微结构，抗ＴＮＦ单克隆抗体（ＴＮＦ－ＭＡｂ）和头孢噻甲羧肟的保护作用，结果显示：急性胆道感染组血浆ＴＮＦ、ＥＴ水平显著升高，血清ＡＬＴ活性也显著升高，肝组织病理变化明显；ＴＮＦ－ＭＡｂ与头孢噻甲羧肟保护组血浆ＴＮＦ，ＥＴ水平及血清ＡＬＴ活性显著下降，肝组织病理变化较轻，结果提示ＴＮＦ是急性胆道感染肝损害的重要介质；ＴＮＦ－ＭＡｂ和头孢噻甲羧肟对胆道感染肝损害有保护作用。

Protective effect of anti-TNF MeAb and ceftazidime on liver injury in acute bile ductinfection.

In this experiment,72 Sprague-Dawleyrats were randomizedly divided into fourgroups,sham operation group,bile duct infec-tion group, and two treatment groups usinganti-TNF McAb and ceftazidime. acute bile duct infection model was made by injecting E.coli to the common bile duct after ligating it.Serum TNF,ET and ALT were determined in all groups, and the liver structures and ultra-structures of liver were also observed. serumTNF. ET level and ALT activity of bile duct infection group rats were significantly higherthan that of sham operation group rats(P<0. 01). The pathological changes of livers wereprominan Treated with anti-TNF McAb andceftazidime, The serum TNF. ET level, and ALT activity of bile duct infection rats di-creased significantly the pathological changesof livers were also milder. TNF is one of the most important mediator for liver Pathologicalinjury. and Anti-TNF McAb and ceftazidimeplay a protective role for liver injury in acutebile duct infectio rats.