In vitro translation of avian erythroblastosis virus RNA: Identification of two major polypeptides

RNA of avian erythroblastosis virus (AEV) pseudotyped with RAV-1, AEV(RAV-1), and its fragments containing poly(A) were translated in an mRNA-dependent reticulocyte lysate. p74 and 46 were found to be AEV RNA specific out of several main products, pr76, p74, p46, and p37. p74 was translated from 30 S AEV RNA and contained some peptides homologous to pr76, but most peptides as specific. On the other hand p46 was translated from 20 S AEV RNA containing poly(A). However, no relation to known viral proteins was established. This preliminary observation suggests that the 5′-half of AEV genome encodes p74 and the 3′-half p46.