| 不同种源高良姜遗传多样性的AFLP分析 |
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| 引用本文： | 杨全,张春荣,陈虎彪,滕希峰,唐晓敏. 不同种源高良姜遗传多样性的AFLP分析[J]. 中国中药杂志, 2011, 36(3): 330-333 |
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| 作者姓名： | 杨全 张春荣 陈虎彪 滕希峰 唐晓敏 |
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| 作者单位： | 1. 广东药学院中药学院,广东广州,510006
2. 香港浸会大学中医药学院,香港 |
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| 基金项目： | 国家科技基础条件平台工作项目(2005DKA21000);国家科技基础性工作专项重点项目(2007FY110600);广东省科技计划项目(2009B060700076);广东药学院博士启动项目;广东药学院重点培养青年教师项目 |
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| 摘 要： | 目的:研究不同种源高良姜的遗传多样性,探求各种质间的亲缘关系,为合理利用高良姜种质资源及优良品种选育奠定理论基础.方法:采用扩增片段长度多态性(AFLP)技术,对8个种源地的高良姜进行遗传多态性分析,将扩增出的条带作为原始矩阵,用NTSYSpc-2.11F软件计算并分析高良姜种质间的相似度,构建遗传系统进化树.结果:通过筛选得到8对AFLP引物组合,共扩增出1120个DNA条带,多态性条带为1044个,多态性位点平均为92.57%;通过构建系统进化树,将8个种源地的高良姜种质分为3类.结论:高良姜种质间存在较高多态性,遗传多样性丰富.
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| 关 键 词： | 高良姜 种质资源 AFLP 遗传多样性 |
| 收稿时间： | 2010-09-19 |
AFLP analysis of genetic diversity of Alpinia officinarum |
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| YANG Quan,ZHANG Chunrong,CHEN Hubiao,TENG Xifeng and TANG Xiaomin. AFLP analysis of genetic diversity of Alpinia officinarum[J]. China Journal of Chinese Materia Medica, 2011, 36(3): 330-333 |
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| Authors: | YANG Quan ZHANG Chunrong CHEN Hubiao TENG Xifeng TANG Xiaomin |
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| Affiliation: | School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;School of Chinese Medicine, Hong Kong Baptist University, Hong Kong,China;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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| Abstract: | Objective : To explore the genetic diversity and relationship of different Alpinia officinarum germplasm. Method : Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic polymorphism in A. officinarum from eight resources. The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A. officinarum germplasm and to construct the genetic phylogenetic tree. Result : A total of 1 120 fragments were genotyped using AFLP with eight prime combinations. Analysis identified 1 044 polymorphic fragments, accounting for 92.57% of the total detected variation. Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A. officinarum. Conclusion : Significant polymorphism and genetic diversity can be observed among A. officinarum germplasm resources. |
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| Keywords: | Alpinia officinarum germplasm resources amplified fragment length polymorphism (AFLP) genetic diversity |
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