IL-18-deficient mice are resistant to endotoxin-induced liver injury but highly susceptible to endotoxin shock |
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Authors: | Sakao Y Takeda K Tsutsui H Kaisho T Nomura F Okamura H Nakanishi K Akira S |
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Institution: | Department of Biochemistry, Hyogo College of Medicine, Nishinomiya, Japan. |
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Abstract: | IL-18 is an IL-1-related cytokine which shares biological functions with IL-12. These include the activation of NK cells, induction of IFN-gamma production and Th1 cell differentiation. In this study we analyzed the effect of IL-18 deficiency on lipopolysaccharide (LPS)-induced liver injury and endotoxin shock in Propionibacterium acnes-primed mice. P. acnes-primed IL-18-deficient (IL-18KO) mice showed resistance to LPS-induced liver injury. Unexpectedly, P. acnes-primed IL-18KO mice were highly susceptible to LPS-induced endotoxin shock. Serum level of tumor necrosis factor (TNF)-alpha were markedly elevated (approximately 10-fold higher) within 1.5 h after LPS challenge in IL-18KO mice as compared with wild-type mice. Anti-TNF-alpha antibody administration to IL-18KO mice was significantly protective against endotoxin-induced lethality. P. acnes-primed IL-18KO macrophages produced approximately 6-fold more TNF-alpha protein than did P. acnes-primed wild-type control macrophages. Taken together, these findings demonstrate that IL-18 is responsible for the progression of endotoxin-induced liver injury as well as down-regulation of endotoxin-induced TNF-alpha production in P. acnes-primed mice. |
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Keywords: | endotoxin shock IL-18 lipopolysaccharide liver injury tumor necrosis factor- " target="_blank">gif" ALT="{alpha}" BORDER="0"> |
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