先天性小肠闭锁Cajal间质细胞和突触素的分布

目的探讨Cajal间质细胞(ICCs)及突触素(SY)在先天性小肠闭锁(IA)发病中的作用。方法应用免疫组化PV法对5例对照者和16例IA患儿肠标本进行ICCs、SY检测。结果在对照组,ICCs大量分布在肌层内及肌间神经丛周围,并形成网络结构,肌间神经丛、纵肌层、环肌层、黏膜肌层均可见大量SY免疫反应阳性产物。在IA组,ICCs和SY在盲端近、远端的分布与正常对照组相比显著减少,ICCs网络被破坏。结论CajalICCs、SY分布异常是IA病理改变之一,可能是该病重要的发病机制,参与肠运动障碍的发生。

A study on the interstitial cells of Cajal and synaptophysin in the patients with congenital intestinal atresia

Objective To investigate the role of interstitial cells of Cajal(ICCs) and synaptophysin(SY) in congenital intestinal atresia (IA). Methods The distribution of ICCs and SY were examined in 16 cases of IA and 5 normal controls by PV immunohistochemistry. Results In bowels from normal controls moderate to large amount of ICCs were observed in between the muscel layers.Myenteric plexuses were clearly demarcated by ICCs.ICCs in both the muscle layers and around the myenteric plexus appeared as a network structure. SY immunoreactivity was abundant in the myenteric plexuses, circular and longitudinal muscle layers and submucosae. On the contrary,the mumber of ICCs was remarkably reduced or even disappeared in the proximal segment,and the network was destroyed.The expression of SY immunoreactivity was remarkably reduced in these segments.Conclusion Abnormal distribution of ICCs and SY in the bowel affected by IA may explain the postoperative intestinal dysmotility in these patients.