机会性筛查人群中高危型人乳头瘤病毒与宫颈病变相关性的分析

目的研究第二代杂交捕获法（HC2）高危型人乳头瘤病毒（HR—HPV）检测阳性样品中HPV与宫颈病变的相关性。方法随机抽取HC2阳性患者602名（344名行细胞学检查）,采用PCR一反向点杂交（RDB）法对HPV分型并分析HPV型别与细胞学的相关性。结果602例患者PCR阳性率94．5％;HPV分型检出率依次为HPVl6、52、58、56、39等;HC2检测谱外单独感染24例;HPVl6、HPVl6／18与ASCUS、LSIL、HSIL有明显相关性。结论机会性筛查人群中最常见HPV型别依次为HPVl6、52、58;HC2检测的假阳性率为4．0％;HPVl6与宫颈病变程度相关。

Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening

Objective To investigate the association between HPV genotypes and cervical lesion in Hybrid Capture 2 ( HC2 ) HPV test positive samples. Methods 602 cervical samples randomly obtained detected as HPV positive by the HC2 high-risk probe cocktail were determined by polymerase chain reaction (PCR)-reverse dot blot (RDB), among them 344 participated Thinprep Cytology test. Results 569 (94. 5% ) samples were successfully amplified. The most common HPV genotypes were HPV16(31.6% ),52 ( 16. 7 % ), 58 ( 15.1% ), 56 ( 8. 1% ), 39 ( 7. 9% ) ; HPV 16 or HPV 16/18 infection was significantly related to ASCUS, LSIL and HSIL; 24 samples suggested single HPV infection with the genotypes not available for HC2. Conclusion The most common HPV genotypes in random screening were HPVI6, 52 and HPV58; Our data demonstrated the pseudo-positivity of HC2 test was 4.0% ; HPV16 was a index for progression of HSIL.