| 自噬在ALA-PDT诱导C6胶质瘤细胞死亡中的作用机制 |
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| 引用本文： | 肖虹,冯华,程茗,李梅,杜鹏,程迎新.自噬在ALA-PDT诱导C6胶质瘤细胞死亡中的作用机制[J].立体定向和功能性神经外科杂志,2009,22(3). |
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| 作者姓名： | 肖虹 冯华 程茗 李梅 杜鹏 程迎新 |
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| 作者单位： | 肖虹,冯华,李梅,杜鹏,程迎新(重庆第三军医大学西南医院神经外科,400038);程茗(第三军医大学大坪医院眼科)
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| 基金项目： | 国家自然科学基金项目，重庆市自然科学基金重点项目 |
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| 摘 要： | 目的 探讨自噬在光敏剂5-氨基乙酰丙酸(aminolaevulinic acid,ALA)介导的光动力治疗(photodynamic therapy,PDT)C6胶质瘤细胞死亡中作用机制.方法 ALA-PDT处理后不同时间,用自噬特异染料MDC和免疫荧光法观察自噬水平变化,Western blotting检测自噬相关蛋白cathepsin B的表达水平.结果 MDC染色和免疫荧光观察可见ALA-PDT后C6胶质瘤细胞内有大量含点状荧光颗粒的自噬泡和自噬体标志物LC3绿色荧光蛋白,Western-blot示自噬相关蛋白cathepsin B表达水平明显升高,其白噬水平随AIA-PDT后时间而逐渐增强.结论 ALA-PDT可诱导C6胶质瘤细胞发生自噬,其诱导胶质瘤细胞发生自噬的机制可能与溶酶体酶cathepsin B大量释放有关.
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| 关 键 词： | 5-氨基乙酰丙酸 光动力治疗 C6胶质瘤细胞 自噬 |
Effects of autophagy on ALA-PDT induced C6 glioma cells death |
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| Xiao Hong,Feng Hua,Cheng Ming,et al..Effects of autophagy on ALA-PDT induced C6 glioma cells death[J].Chinese Journal of Stereotactic and Functional Neurosurgery,2009,22(3). |
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| Authors: | Xiao Hong Feng Hua Cheng Ming |
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| Institution: | Xiao Hong,Feng Hua,Cheng Ming,et al.Department of Neurosurgery,Southwest Hospital,The Third Military Medical University,Chongqing,400038,China. |
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| Abstract: | Objective To study the effects and the mechanisms of autophagy on photosensitizer Aminolaevulinic acid(ALA)-mediated-photodynamic-therapy induced death of C6 glioma cells.Methods After treatment with ALA-PDT,MDC staining and immunofluorescence were used to examine autophagy induced by ALA-PDT in C6 glioma cells.The expression levels of autophagy-associated-protein cathepsin B were measured by Western blot.Results ALA-PDT stimulated the autophagic vacuole and LC3 activity as evidenced by the appearance of pu... |
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| Keywords: | Aminolaevulinic acid Photodynamic therapy C6 glioma cells Autophagy |
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