| 利用荧光探针Fluo-4检测胸腺细胞内钙离子浓度变化 |
| |
| 引用本文： | 于力方,廖杰,张国庆. 利用荧光探针Fluo-4检测胸腺细胞内钙离子浓度变化[J]. 标记免疫分析与临床, 2005, 12(3): 161-163 |
| |
| 作者姓名： | 于力方 廖杰 张国庆 |
| |
| 作者单位： | 解放军总医院医学实验测试中心,北京,100853;解放军总医院医学实验测试中心,北京,100853;解放军总医院医学实验测试中心,北京,100853 |
| |
| 摘 要： | 利用荧光显微数码成像系统测量荧光探针Fluo-4荧光强度的改变,建立动态检测细胞内钙离子浓度的方法.用荧光探针Fluo-4/AM标记原代培养小鼠胸腺细胞,以KCL刺激胸腺细胞去极化,并打开细胞膜上电压依赖性钙通道,细胞内荧光强度会发生改变.利用荧光显微数码成像系统动态监测Fluo-4荧光强度的改变可分析计算钙离子浓度.本方法灵敏度高,能实时监测细胞内钙离子浓度的变化.
|
| 关 键 词： | 荧光探针Fluo-4 荧光显微镜 钙离子 |
| 文章编号： | 1006-1703(2005)03-0161-03 |
| 收稿时间： | 2005-04-07 |
| 修稿时间： | 2005-04-25 |
A Method of Measuring Intracellular Free Calcium in Thymocytes by Fluorescence Probe Fluo-4 |
| |
| YU Li-fang,LIAO Jie,ZHANG Guo-qing. A Method of Measuring Intracellular Free Calcium in Thymocytes by Fluorescence Probe Fluo-4[J]. Labeled Immunoassays and Clinical Medicine, 2005, 12(3): 161-163 |
| |
| Authors: | YU Li-fang LIAO Jie ZHANG Guo-qing |
| |
| Affiliation: | Medical Experiment and Analysis Center, PLA General Hospital, Beijing 100853, China |
| |
| Abstract: | To explore the kinetic changes in measuring calcium in thymocytes with fluorescence probe Fluo-4 by fluorescence micro-imaging system and establish a method of measuring the quantity variation of intracellular calcium, thymocytes were cultured and stimulated with KCL. After stimulated by KCL, the voltage-dependent calcium channels were opened and the fluorescence intensity in thymocytes was changed. Our experience is that using fluorescence microscopy and digital CCD to measure the quantity variation of intracellular free calcium is highly sensitive for real-time monitoring. |
| |
| Keywords: | Fluorescence probe Fluo-4 Fluorescence microscopy Free calciumion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
