肿瘤坏死因子受体实验研究

应用本室制备的肿瘤坏死因子生物素（ＴＮＦＢ），藉ＡＢＣ法对几种肿瘤细胞坏死因子受体（ＴＮＦＲ）进行定位分析，结果与文献报道的放射受体结合分析法一致，３株胃癌细胞ＴＮＦＲ存在情况与ＴＮＦ对这３株胃癌细胞的抑制率相吻合。应用荧光分光光度法测定胃癌细胞内游离钙浓度（［Ｃａ２＋］ｉ），ＴＮＦ可引起胃癌细胞ＦＧＣ－８５的［Ｃａ２＋］ｉ急性升高，提示ＴＮＦ对［Ｃａ２＋］ｉ有明显影响。

An Experimental Study on Tumor Necrosis Factor Receptor

Tumor necrosis factor(TNF)was prepared and purified by the established method of our department.The purified TNF was labelled with biotin as biotinylated TNF(TNFB).With TNFB and ABC reagent,the localization and distribution of TNF receptor(TNFR)of several cell lines in smears were assaied. The result was in agreement with that of the radioreceptor assay.The existence of TNFR was consistent with the inhibitory rate by TNF on three gastric carcinoma cell lines.Intracellular free calcium(Ca2+]i)was examined by spectrofluorimeter.The results showed that TNF was abruptly increased in Ca2+]i in gastric carcinoma cell line FGC-85,suggesting thatCa2+]i was influenced by TNF.