CD44v6、MMP-9与VEGF的表达与胃癌生物学行为的关系

目的:探讨粘附分子CD44拼接变异体v6(CD44 splice variant v6,CD44v6)、基质金属蛋白酶-9(matrix metallopro-teinase-9,MMP-9)与血管内皮生长因子(vascular endothelia1 growth factor,VEGF)的表达与胃癌临床病理生物学行为的关系以及它们之间的相关性。方法:应用免疫组化SP法对80例胃癌组织进行CD44v6、MMP-9与VEGF蛋白检测。结果:CD44v6、MMP-9与VEGF的表达与胃癌的临床分期、浸润深度、淋巴结转移及远处转移密切相关(P<0.05),而与组织类型、组织分化程度等无关;CD44v6、MMP-9与VEGF在胃癌组织中表达呈正相关,其共同表达者发生淋巴结转移与深度浸润的几率明显高于其中之一或之二表达者或三者皆阴性表达者(P<0.05)。结论:CD44v6、MMP-9与VEGF的表达促进胃癌的浸润转移,三者具有正协同作用。联合检测CD44v6、MMP-9与VEGF蛋白可作为预测胃癌的浸润转移及评价患者预后的生物学指标。

Correlation Between the Expression of CD44v6, MMP-9 and VEGF and the Biological Behavior in Gastric Carcinoma

Objective:To investigate the association of the expression of CD44v6,MMP-9 and VEGF(CD44 splice variant v6,matrix metalloproteinase-9 and vascular endothelial growth factor) with the clinicobiologic behavior in gastric carcinoma and the correlation between the 3 factors.Methods: The expression of CD44v6,MMP-9 and VEGF in 80 cases of gastric carcinoma tissue were detected by immunohistochemical streptavidin peroxidase.Results: The expressions of CD44v6,MMP-9 and VEGF were positively related to the clinical stage,the depth of invasion,lymph node metastasis and distant metastasis and were synergistic in action with one another(P<0.05),but was not related to the degree of histological differentiation and the type of tissue.The probability of lymph node metastasis and deep invasion was higher in the patients with over expression of the 3 factors than in those patients with over expression of 1 or 2 factors or with negative expression of the 3(P<0.05).Conclusion: The expressions of CD44v6,MMP-9 and VEGF are correlated to invasion and metastasis of gastric carcinoma,and thus can be as markers to predict metastasis and invasion of gastric carcinoma and the prognosis of these patients as well.