Solid-phase radioimmunoassay for hepatitis be antigen (HBeAg) |
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| Authors: | H. E. Blum G. Dölken Prof. Dr. W. Gerok |
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| Affiliation: | (1) Medizinische Klinik der Universität Freiburg i. Br., Germany |
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| Abstract: | Summary A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.
Abbreviations HBsAg hepatitis B surface antigen - HBeAg hepatitis Be antigen - HBe/sAg hepatitis Be antigen and surface antigen - anti-HBe antibody to hepatitis Be antigen |
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| Keywords: | Hepatitis B HBsAg HBeAg anti-HBe Solid-Phase Radioimmunoassay |
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